利洛司酮对子宫内膜异位症大鼠血清黄体生成素和雌二醇水平的影响

Effect of lilopristone on serum levels of luteinizing hormone and estradiol in experimental endometriosis rats

目的 :观察利洛司酮对子宫内膜异位症大鼠血清黄体生成素 (LH)和雌二醇 (E2 )水平的影响。方法 :2 0只子宫内膜异位症大鼠 ,随机分成 4组 ,灌胃给予利洛司酮 2 0 ,80mg·kg- 1,米非司酮 5 0mg·kg- 1和精制花生油 4mL·kg- 1,每日 1次 ,共 2 1d。采集用药d 19,2 0 ,2 1及停药 2 4h的血样本 ,用大鼠睾丸间质细胞睾酮法 (RICT法 )及放射免疫分析(RIA)法分别测定血清LH和E2 的水平。结果 :对照组大鼠给药d 19,2 0 ,2 1及停药 2 4h ,这 4d的血清LH和E2 水平均有周期性变化 ,出现一个动情前期的LH峰和E2 峰 ;利洛司酮 2 0 ,80mg·kg- 1及米非司酮 5 0mg·kg- 1组大鼠血清LH和E2 水平未见周期性变化 ,也未见动情前期的LH峰和E2 峰。结论 :和米非司酮一样 ,利洛司酮可抑制LH和E2 的分泌 ,取消动情前期的LH峰和E2

AIM: To observe the effect of lilopristone (Lil) on the serum levels of luteinizing hormone (LH) and estradiol (E 2) in the rat of experimental endometriosis. METHODS: Twenty rats of the experimentally induced endometriosis were randomly divided into 4 groups. They were daily given intragastrically with Lil 20 mg·kg -1, 80 mg·kg -1, mifepristone (Mif) 50 mg·kg -1 and peanut oil 4 mL·kg -1 for 21 d, respectively. Blood specimens were collected in the morning of d 19, 20, 21 of giving the drugs and 24 h after withdrawal. Serum LH activity was determined by the rat interstitial cell testosterone (RICT) assay, and serum E 2 level was measured by specific radioimmunoassay. RESULTS: In control rats, cyclical changes in serum LH and E 2 levels were shown, the proestrus LH and E 2 surge appeared. In the rats treated with Lil 20, 80 mg·kg -1 and Mif 50 mg·kg -1, cyclical changes in serum LH and E 2 levels appearing in the control rats disappeared, and the proestrus LH and E 2 surge were abolished. CONCLUSION: As Mif, Lil could inhibit the secretion of LH and E 2, and abolish the proestrus LH and E 2 surge.