青霉植酸酶生产条件及纯化的研究

Studies on the Condition of Phytase Produced by Penicillium and Its Purification

青霉培养液的原始pH值为 5 .5时 ,植酸酶大量地产生 ,酶活性高 .在青霉的生长过程中 ,维持培养液pH的不降低会抑制青霉的生长 .植酸钙可促进植酸酶活性的提高 .粗酶液经硫酸铵分级沉淀 ,再经SephadexG - 10 0凝胶过滤 ,洗脱液有 2个具有酶活力的蛋白峰 ,植酸酶被纯化了 3.3倍 ,比活力达 71.3U/mg.

When the original pH value of the penicillium's culture fluid was 5.5,the phytase was produced much .While the pH of culture fluid didn't decrease, the growth of penicillium could be inhibited. Calcium phytate could promote the penicillium to increase phytase activities.Two isoenzymes of phytase from penicillium were accepted by ammonium sulfate precipitation,sephadex G-100 gel filtration chromatography. The purification factors of phytase reached 3.3 folds with a specific activity of 71 3 U/mg.