摘要
报道了广东南昆山和始兴都亨、广西靖西三地的观光木Tsoongiodendronodorum居群基因多态性研究的初步结果 .应用随机扩增多态DNA(RAPD)分子标记方法 ,从 10 8个 10个碱基的随机引物中获得了 18个多态性标记 ,在 3个居群共 4 2个样株中 ,共检测到 78个位点 ,其中多态位点 6 6个 ,占 84 .6 2 % ,显示该物种的基因多样性较高 .聚类分析结果表明 ,样品可分为 3个居群 ;广东境内的 2个居群间相似性较高 ,相似性系数达 0 .832 ,广西与广东都亨和南昆山 2个居群间的相似性较低 ,相似性系数分别为 0 .772和 0 .76 8.南昆山居群内的基因多样性最高 ,广东始兴都亨居群的基因多样性最低 。
The preliminary results on genetic diversity of Tsoongiodendron odorum at three sites of southern China were described. Eighteen 10 mer primers selected from 108 were applied for RAPD analysis. Seventy-eight genes were obtained from 42 leaf samples from the three populations, 66 of them (84.62% of total genes) were polymorphic. The result of cluster analysis showed that the population of Nankunshan of Guangdong province had the highest genetic diversity (3.074), the second was Jingxi of Guangxi province(2.389), the least was Duheng of Guangdong province (2 195). The similarity index between Nankunshan and Duheng in Guangdong province was the highest (0.832), that of Jingxi and Duheng was 0 772, and the similarty index between Jingxi and Nankunshan was 0 768.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2002年第2期54-57,共4页
Journal of South China Agricultural University
基金
香港嘉道理暨植物园生物多样性奖学金 (G0 0 0 0 3)
