突变系无毛小鼠近交系的繁殖性能观察

Study on reproductive performance for hairless mice of inbred strain

目的 :观察突变系无毛小鼠近交系的繁殖性能 ,找出最佳保种方式。方法 :从 2 1代到 2 9代分别采用无毛纯合子雄性与有毛杂合子雌性、有毛杂合子雌雄、无毛纯合子雌雄、有毛杂合子雄性与无毛纯合子雌性 4种交配方式 ,对其生产胎数、胎平均产仔数、离乳数、无毛小鼠的生产机率等进行观察统计。结果 :前 2种交配方式 ,生育小鼠中均有无毛小鼠 ,生产胎数以 2或 3胎为主 ,平均产仔数和平均离乳率无明显差异 ,但无毛纯合子雄性与有毛杂合子雌性交配 ,无毛小鼠的生产机率明显高于有毛杂合子雌雄交配 (P <0 .0 1)。后 2种交配方式 ,无毛纯合子雌性受孕较难 ,即使受孕 ,所产仔鼠于生后不久全部死亡 ,无一存活至离乳日龄。结论 :无毛突变系的繁殖保种最好采取无毛基因纯合的雄性 (具有繁殖力 )和有毛杂合子的雌性交配。

Aim:To study the reproductive performance for hairless mice of inbred strain and find out the best way to keep this mutant strain. Methods: The 4 different mating patterns were adopted. The average fetus number, average whelp number, average weaning rate had been investigated from 21 to 29 generations. Results:For forced crossing of heterozygous hairy males and females or forced crossing homozygous hairy males and females,there was significant difference in average hairless fetus number but not in average whelp number or average weaning rate . If forced crossing of hairless males and hairless females or heterozygous males and hairless females, the females were difficult to be pregnant and even if pregnant,all the born babies would die before weaning day.Conclusion: The best way to keep this mutant hairless strain should be forced crossing between homozygous hairless males and heterozygous females inbred.