退变腰椎间盘组织中碱性成纤维细胞生长因子的表达研究

The study of expression of the basic fibroblast growth factor in normal and degenerated human intervertebral disc

目的探索碱性成纤维细胞生长因子(basicfibroblastgrowthfactor,bFGF)在正常和退变椎间盘组织中的表达情况。方法将30例来源于腰椎间盘突出症患者手术中所取得的椎间盘组织(观察组,男11例,女19例;年龄25～78岁,平均48岁;病程3个月～30年,平均9年11个月)与6例来源于脊柱侧凸患者前路松解术所取得的椎间盘组织(对照组,男女各3例,年龄10～17岁,平均14.2岁)进行对比。首先经病理组织学检查证实为退变椎间盘组织和正常椎间盘组织,然后将两组椎间盘组织分别通过免疫组织化学方法和原位杂交方法,检测各自椎间盘组织中的bFGF及其mRNA的表达。结果观察组30例均为退变椎间盘组织,免疫组化阳性率为90%(27/30),原位杂交阳性率为20%(6/30);对照组6例均为正常椎间盘组织,其免疫组化及原位杂交均为阴性。两组间免疫组化方法检测阳性率在统计学上差异有非常显著性意义。结论bFGF在正常和退变椎间盘组织中表达的差异有显著性意义,提示bFGF可能作为增生刺激因子促进椎间盘组织中的软骨细胞增生和细胞外基质合成,进而加速椎间盘退变。

Objective To investigate the expression of the basic fibroblast growth factor (bFGF) in normal and degenerated human intervertebral discs in order to determine whether bFGF is related to degeneration of the intervertebral disc. Methods The specimens of the intervertebral discs from 11 male and 19 female patients undergone lumbar disc herniation surgery (observation group) and 6 patients with scoliosis following anterior release (control group) were harvested. The tissues were histologically observed to confirm their degenerated or normal status and then conducted for the expression of bFGF and its mRNA with immunohistochemistry and in situ hybridization. Results In the observation group, all the samples were found to be degenerated disc tissue, and the positive rate of the expression of bFGF was 90% (27/30) with immunohistochemistry and 20% (6/30) with in situ hybridization. In the control group, all the samples were shown to be normal disc tissues, and had negative expression of bFGF with immunohistochemistry and in situ hybridization. The positive rate of the expression of bFGF showed significant difference between the two groups. Conclusion The expression of bFGF showed significant difference between the degenerated and normal intervertebral discs, which indicated that the bFGF might promote proliferation of chondrocyte and synthesis of extracellular matrix in the degenerated discs as a proliferation stimulating factor.