银屑病患者外周血单核细胞向树突状细胞分化能力的研究

Differentiation of Monocyte derived Dendritic Cells in Patients with Psoriasis

目的了解银屑病患者外周血单核细胞向树突状细胞(DC)分化的能力。方法采用流式细胞仪检测细胞表面抗原表达,同种混合淋巴细胞反应评价细胞的抗原递呈能力。结果银屑病患者外周血单核细胞能向DC分化,分化而成的DC表达CD40、CD80、CD86和HLA-DR的阳性率显著高于正常人(P<0.01),刺激淋巴细胞增殖的能力显著强于正常人(P<0.01)。结论银屑病患者外周血单核细胞向DC分化的能力增强,分化而成的DC具有很强的抗原递呈能力。

Objective To investigate the differentiation of dendritic cells from monocytes of the peripheral blood in patients with psoriasis. Methods Flow cytometry was used to analyze the phenotype of monocyte derived dendritic cells (MoDC). The capacity of MoDC to stimulate the proliferation of T lymphocytes was evaluated by allogeneic mixed lymphocyte reaction. Results Monocytes in the peripheral blood of patients with psoriasis could differentiate into dendritic cells. Expression of CD40, CD80, CD86 and HLA-DR by MoDC was significantly increased in patients with psoriasis compared with that in normal controls (P< 0.01). The capacity of MoDC to stimulate T lymphocytes was enhanced significantly in patients′group as well (P< 0.01). Conclusions The differentiation of monocytes into dendritic cells is upregulated in psoriasis, and these cells have potent antigen presenting capacities.