病毒性心肌炎小鼠体内γ-IFN与NO相关性研究

Correlative study between γ-IFN and NO in mice with viral myocarditis

为探讨γ -IFN、NO在实验性病毒性心肌炎小鼠中的变化及其在病毒性心肌炎中的作用 ,以柯萨奇病毒B3亲心肌株(CVB3m)诱导的BALB/C心肌炎小鼠为研究对象 ,每隔7天观察小鼠血清中γ -IFN、心肌匀浆中NO在病程中的动态变化 ,并计算感染小鼠心肌组织光镜下病理积分。结果显示 ,BALB/C鼠感染病毒后体内γ -IFN、NO均有不同程度升高 ,急性期(第7天)最高 ,且NO的升高与γ -IFN相关(r=0.95,r2=0.9,P<0.05) ,而感染后心肌病理积分又与NO相关(r=0.66,r2=0.43,P<0.05)。结果表明 ,γ -IFN在病毒性心肌炎发病中起重要作用 ,介导NO产生以发挥抗病毒作用 ,而过分表达则可造成自身损伤 。

The present study tried to explore the change of serum γ-IFN and NO content in myocardial tissue in BALB/C mice with viral myocarditis which was created by the injection of 0.3ml VCB3m intraperitoneally.The contents of serum γ-IFN and myocardial NO in BALB/C mice were determined by ELISA and nitric acid reductase methods at 7,14 and 21 days after the injection of VCB3m,respectively.The pathological changes of myocardial tissue were also assessed in the corresponding time.The results showed that γ-IFN and NO levels increased in different degree,in particular there was a peak in the seventh day.The positive correlations either γ-IFN(r=0.9～0.95)or pathological change(r=0.43～0.66,p<0.05)with NO were found in BALB/C mice with viral myocarditis. It is suggested that γ-IFN,which can induce the production of NO,play an important role in the antiviral effect.However,overexpression of γ-IFN and NO can result in self_damages,it will be clinically helpful to decrease their levels eligibly.