谷胱甘肽转硫酶T1、M1基因型和烟酒茶嗜好与食管癌、胃癌

Effects of GSTT1 and GSTM1 Genotypes,Lifestyle Factors and Their Interactions on Risk of Esophageal and Stomach Cancers

目的 :研究谷胱甘肽转硫酶T1、M1(GSTT1、GSTM 1)基因多态性和烟酒茶嗜好及其相互作用与食管癌、胃癌易感性的关系。方法 :在上消化道癌高发区淮安市进行了病例 -对照研究 (食管癌 141例 ,胃癌 15 3例 ;人群对照 2 2 3例 ) ,调查研究对象的烟酒茶嗜好习惯 ,以多重PCR方法分析GSTT1、GSTM1基因型。结果 :食管癌组GSTM1-基因型频度 (75 .18% )显著高于对照组 (5 9.6 4 % ,P =0 .0 0 2 4 ;多因素调整OR =2 .33,95 %CI =1.39～ 3.92 )。吸烟或不饮茶与GSTM 1 基因型在增加食管癌发生的风险中有明显的协同作用。在GSTT1+基因型者中 ,吸烟习惯显著增加食管癌、胃癌的危险性 ;在GSTM1+基因型者中 ,经常饮酒显著增加食管癌、胃癌的危险性。结论 :食管癌、胃癌的发生与生活习惯、GSTM1和GSTT1基因型以及它们的相互作用有关。

Objective To evaluate effects of GSTM1 and GSTT1 genotypes,lifestyle factors and their interactions on risk of esophageal and stomach cancers.Methods A case control study consisted of 141 esophageal cancer patients,153 stomach cancer patients,and 223 population based controls was conducted in Huaian,Jiangsu province,China.The epidemiological data and DNA of peripheral blood leukocytes was obtained from all of the subjects.GSTM1 and GSTT1 genotypes were determined by a multiplex PCR.Results The frequency of the GSTM1 null genotype was found to be significantly higher among esophageal cancer than that among controls(75.18% vs 59.64%, P =0.002 4),with an adjusted OR of 2.33(95%CI=1.39-3.92).A significant interaction was observed between cigarette smoking or tea drinking and the GSTM1 null genotype with respect to OR for esophageal cancer.As compared with non smokers with the non null GSTM1 genotype,the OR for esophageal cancer among smokers with the null GSTM1 genotype was increased to 5.08(95%CI=2.12- 12.15 ).As compared with tea drinkers having the non null GSTM1 genotype,the OR for esophageal cancer among non tea drinkers with the null GSTM1 genotype was also increased to 4.86(95%CI=1.89-12.50).On the other hand,smoking significantly increased the OR for esophageal or stomach cancers among subjects with GSTT1 non null genotype.Alochol drinking significantly increased the OR for esophageal or stomach cancer among subjects with non null GSTM1 genotype. Conclusions Our results in the present study show that the interactions between the GSTM1,GSTT1 genotypes and encvironmental factors are important in the development of cancers of the esophagus and the stomach.