蝎毒抗癌多肽对肝癌小鼠放疗后瘤重和血液系统的影响

The Influence of Antineoplastic Polypeptide from Buthus Martensii Venom on Tumor Weight and Hematological System of Hepatoma-bearing Mice After Radiotherapy

目的 :观察蝎毒抗癌多肽 (APBMV)与放疗 (RT)合用对H2 2 带瘤小鼠瘤重和血液系统的影响。方法 :取H2 2 带瘤小鼠 10 0只 ,检测肿瘤生长抑制率 (IR)、白细胞计数、脾脏指数 (SI)和外周血血红蛋白含量 ,观察不同剂量APBMV与放疗合用后上述指标的变化。结果 :放疗后第 6天和第 9天 ,RT与APBMV合用后瘤重明显降低 ,IR最高达 78 2 8%和 70 4 5 % (与RT组和APBMV组比较 ,P <0 0 5或P <0 0 1) ;白细胞计数和SI较RT组显著升高 (P <0 0 5 ) ;血红蛋白含量测定结果各组间差异无显著性 (P >0 0 5 )。结论 :APBMV与放疗合用对H2 2 的抑制作用比单纯放疗和单用APBMV增强 。

Objective To observe the effect of antineoplastic polypeptide from Buthus martensii venom (APBMV) combined with radiotherapy on tumor weight and hematological system of Hepatoma 22 bearing mice.Methods One hundred H 22 bearing mice were used in this experiment.The tumor growth inhibiting rate (IR%),WBC count,spleen index (SI) and hemoglobin were used as the parameters.After radiotherapy (RT) or after administration of different dosage of APBMV combined with RT,the changes of these parameters were observed.Results On the 6th day and 9th after radiotherapy,the tumor weights decreased after administrating APBMV combined with RT,in which IR were 78 28% and 70 45%,respectively (comparing with RT and APBMV group, P <0 05 or P <0 01).In RT+APBMV group,WBC count and SI were also higher than those of the RT group ( P <0 05).The results of hemoglobin content in peripheral blood indicated that there were no differences among all groups ( P >0 05).Conclusions The inhibiting effect of the combining therapy (APBMV combining with RT) on H 22 was stronger than radiation or APBMV alone.APBMV can decrease the hematological system injury induced by radiation in some extent.