有丝分裂激活蛋白激酶激活与前列腺癌的恶化进展

Activation of Mitogen Activated Protein Kinase(MAPK) and Progression of Human Prostate Carcinoma

目的 :研究人前列腺癌组织中有丝分裂激活蛋白激酶 (MAPK)激活和前列腺癌发生、恶化进展的关系。方法 :采用免疫组织化学分析方法 ,用抗磷酸化MAPK蛋白的抗体检测 4 8例人前列腺癌组织标本中MAPK激活。结果 :所有前列腺癌组织中均有磷酸化MAPK蛋白表达 ,而非癌前列腺组织中未发现有磷酸化MAPK蛋白表达。磷酸化MAPK蛋白表达强阳性在Gleason分值≤ 7和 8～ 10组中分别为 2 0 %和 6 1.1% (P <0 .0 1) ;在局部浸润性肿瘤 (T3 期 )中强阳性率 5 7 1%明显高于局限性肿瘤 (T1～T2 期 ) 2 6 5 % (P <0 .0 1)。结论 :MAPK激活和人前列腺癌的发生及恶化进展密切相关 ,可以作为估价前列腺癌恶性程度和预后的指标。

Objective To investigate the activation of mitogen activated protein kinase(MAPK) in prostate carcinoma tissues.Methods In 48 human prostate carcinoma specimens,the activation of MAPK was recognized by antibody specifically against phosphorylated MAPK protein by using immunohistochemistry method.Results Activation of MAPK was found in all carcinoma tissues,whereas no expression of phosphorylated MAPK protein was detected in noncarcinoma tissues.Intensive expression of phosphorylated MAPK protein was found in 11 cases with Gleason scores 8-10(61 1%) and 6 cases with Gleason score 7 or less than 20%( P <0 01).Intensive expression of phosphorylated MAPK protein in localized tumors(T 1-T 2) 57 1% is much higher than in localized invasive tumors(T 3)26 5%( P <0.01).Expression of phosphorylated MAPK protein is not associated with the level of serum prostate specific antigen.Conclusions Activation of MAPK may play a strong role in the development and progression of human prostate carcinoma.Hence,intensive expression of phosphorylated MAPK protein may be used as a tumor marker evaluating the histograde,clinical stage and prognosis.