转移性恶性肿瘤患者外周血单个核细胞某些生物学特性的比较

Comparasion of Biological Characteristic of Peripheral Blood Monocytes in Patients with Malignant Tumors Between Hematogenous and Lymphatic Metastases

目的 :研究血行转移和淋巴结转移恶性肿瘤患者外周血细胞生物学特性的区别。方法 :用流式细胞术对 32 2例血行转移和 2 0 1例淋巴结转移肿瘤患者外周血细胞DNA倍体、细胞凋亡水平和增殖活性进行了对比分析。结果 :血行转移肿瘤患者外周血细胞DNA异倍体检出率、细胞凋亡水平和细胞增殖活性均显著高于淋巴结转移者 ,其检出率及平均值分别为 12 73%、(3 5 7± 2 14) %、(1 2 4±1 0 6 ) %和 1 0 0 %、(1 94± 1 0 9) %、(0 81± 0 5 3) % (P <0 0 5 )。结论 :血行转移和淋巴结转移肿瘤患者血细胞某些生物学特性有明显不同。这可能为恶性肿瘤血行转移的诊断提供参考依据。

Objctive To study difference of bilolgical characteristic in peripheral blood monocytes on patients with malignant tumors between hematogenous and lymphatic metastases.Methods DNA ploidy,apoptosis level and proliferative activity of peripheral blood monocytes in 322 patients with hematogenous metastases and 201 patients with lymph node metastases were measured and compared by flow cytometry.Results Positive rate of DNA aneuploidy,apoptosis level and proliferative activity of patients with hematogenous metastases were higher than those of patients with lymph node metastases.The above targets in these patients were 12 73%,(3 57±2 41)%,(1 24±1 06)% and 1 00%,(1 94±1 09)%,(0 85±0 53)% respectively( P <0 05).Conclusion Biological characteristic in peripheral bolld monocytes were significantly different between hematogenous and lymphatic metastases patients with malignant tumors.The reference basis were provided for determination of hematogenous metastases of malignant tumors.