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HPVI6L1晚期蛋白的鉴定及电镜观察的研究 被引量:2

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摘要 目的:通过对HPVI6L1晚期蛋白的鉴定及电镜观察,为制备宫颈癌预防性疫苗奠定基础。方法:采用Westernblot方法对所表达蛋白进行特异性鉴定,并利用透射电镜观察HPVI6L1在酵母菌中自我组装成的病毒样颗粒(VLPs)。结果:所表达的外源蛋白能与鼠抗HPVI6L1单克隆抗体特异结合,证明该蛋白确为HPVI6L1基因编码,电镜下观察到了HPVI6L1的VLPs。结论:HPVI6L1基因经诱导可表达HPVI6L1晚期蛋白,该蛋白可自我组装成VLPs.
出处 《中国优生与遗传杂志》 2002年第2期28-29,共2页 Chinese Journal of Birth Health & Heredity
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  • 1于修平,卞继峰.人乳头瘤病毒研究的新进展[J].中国病毒学,1996,11(4):301-312. 被引量:2
  • 2Marle L, Annie C, Sue E C, et al . Expression of rotavirus VP2 produce empty corelike particles[J] .J Virol, 1991,65(6) :2946-2952.
  • 3Sue E,Crawford,M L,Jean C, et al .Characterization of virus-like particles produced by the expression of rotavirus capsid protein in insect cells[J] .J Virol, 1994,68(9):5945-5952.
  • 4Carl Q-Y Z, Melissa J W, Jean C, et al . Characterization and replicase activity of double-layered and single-layered rotavirus-like particles expressed from baculovirus recombinants[J] .J Virol, 1996,70(5): 2736-2742.
  • 5Denise N-H, Richard R, Carole B, et al . Mucosal but not parenteral immunization with purifies human papillomavirus type 16 viruslike particles induces neutralizing titers of antibodies throughtout the estrous cycle of mice[J] .J Virol, 1999,73(11) :9609-9613.
  • 6Catherine D, Dominious B G, Antoine T, et al . Nasal Immunization of mice with human papilloma-virus type 16(HPV-16) virus-like psrticles or with the HPV-16 L1 gene elicits specific T lymphocytes in vaginal draining lymph nodes[J] .J Virol, 1999,73(11) :9063-9071.
  • 7Sabine W, Bettina K, HeidiB, et al .Binding of hepatitis C viruslike particles derived from infectious clone H77C to defined human cell lines[J] .J Virol,2002,76(3):1181)1193.
  • 8Marc C J, Heather M S, Volker M V. PR domain of rous virus Gag causes an assembly/budding defect in insect cells [J] .J Virol,2001,75(9) :4407-4412.
  • 9Sayuri S, Toshiyuki G, Kouichi S, et al. HIV type 1 Gag viruslike particle budding from spheroplasts of Saccharomyces cerevisiae [J] .PNAS,2002,99(12) :7956-7961.
  • 10M, Joshi, Vlker M V. Role of the rous sarcoma virus p10 domain in shape determination of Gag virus-like particles assembled In vitro and within E.coli[J] .J Virol,2000,74(21):10260-10268.

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