FPC恶变过程中电镜下凋亡细胞的形态学变化

Change of electron-microscopy morphology of apoptosis cell in the process of FPC canceration and evolution

目的研究家族遗传性大肠息肉病(FPC)恶变演变过程中凋亡细胞形态学特点。方法采用电镜对14例恶变、未恶变FPC和未癌变非遗传性腺瘤性息肉的凋亡细胞形态学变化进行对比研究。结果FPC凋亡细胞与周围细胞连接疏松,其内散在圆形或椭圆形空泡样结构,核内染色质离散,呈均质样或团块样浓集于核膜内侧,核仁缺如,整个细胞核呈固缩状态,核膜与胞质之间界线清晰,并有环形透亮区包绕核膜。不同表现:FPC癌变组织、未癌变息肉和未癌变非遗传性腺瘤性息肉组凋亡细胞数目呈逐渐增多状;FPC未恶变息肉和未癌变非遗传性腺瘤性息肉凋亡细胞早期表现多较规则,多呈球或椭圆形,染色质浓聚、边聚明显,透明区透亮度大,FPC癌变组织凋亡细胞早期多不规则,染色质聚、边聚现象较不规则,透亮区透亮度较低。结论FPC恶变演变的不同过程中非遗传性腺瘤性息肉的电镜超微形态不同,因而电镜观察对诊断良恶性肿瘤有重要参考作用。

Objective To study the process of canceration and evolution in familial polyposis of the colorectum.Methods Electroscopy were used to detect and study14samples of FPC,and compared with microscopy polyposis of adenoma.Results The apopto sis cell was in the state of solidification and atrophy,it was connected loosely with its adjacent cells,dispersing chromatin gathered along the inside of the nuclear membrane.The dividing line between the nuclear membrane and the cytosol was clear,cell organ of cytoplasm gathered together,the rinal cell of apoptotic body collapsed.The apoptosis cell increased gradually from FPC of canceration to FPC of non-canceration.Apoptosis cell of non-cancera-tion's FPC and non-canceration's polyposis of canceration's FPC was quite regular whose morphology was spherical or elliptic,it was quite clear,its chromatin gathered along the inside of the nuclear mem-brane,transparency of bright area was relatively high.Cancerated FPC was different ,apoptosis cell was quite irregular,it was quite not clear,its chromatin gathered along.The inside of the nuclear mem-brane,transparency of bright area is relatively low.Conlu sion Change s of morphology under electron-microscopy are different in the various process of FPC canceration,it is important to differentiating ma-lignant tumour from benign one.