壳聚糖固定化木瓜蛋白酶的研究

Study on Immobilization of Papain on Chitosan

以壳聚糖为载体,戊二醛作为交联剂,采用载体交联法制备固定化木瓜蛋白酶,并研究了固定化木瓜蛋白酶的最佳固定化条件。结果表明:木瓜蛋白酶的最佳固定化条件为给酶量为40～50mg/g,于pH7.5,25～30℃下,0.4%～0.5%的戊二醛溶液交联12h,所得的固定化木瓜蛋白酶的活力回收率平均达61.6%。

Using chitosan as support, glutaraldehyde as crosslinking agent, immobilization of papain were prepared by carrier-crosslinking method. The optimum conditions for immobilization were studied. The results were as follows: the optimum load of enzyme was 40～50mg/g carrier, crosslinking for 12h with 0.4%～0.5%glutaraldehyde at 25～30℃and pH7.5. The activity recovery of the immobilizated papain was 61.6%respectively.