生长抑素pcDNA3s/SS质粒的构建与鉴定

Construction and Identification of Somatostatin pcDNA3s/SS Plasmid

动物生长抑制激素 (Somatostatin ,SS)基因克隆至含有乙肝表面抗原的 pcDNA3s质粒载体上 ,生长抑素基因插入到乙肝表面抗原的 5′末端 ,构建生长抑素DNA疫苗 .经PCR反应扩增出含有SS和HBsAg的融合基因片段 ,并经测序证实SS基因已成功重组到

The animal somatostatin(SS,14AA) was cloned into the pcDNA3s vector which contained hepatits B surface antigen gene(HBsAg gene). The somatostatin gene was inserted at the 5′ end of HBsAg gene. Then the somatostatin DNA vaccine was constructed. The fused gene of SS and HBsAg was amplified through PCR. It was approved that the SS gene had been successfully cloned into pcDNA3s vector after sequence examination.