肝癌单克隆抗体HAb_(23)免疫组化及免疫电镜定位的研究

Studies on Immunohistochemical and Immuno-electron Microscopic Localization of Monoclonal Antibody against Human Hepatocellular Carcinoma HAb23

本研究应用经人α-干扰素处理的肝癌细胞系SMMC-7721作为免疫原,制备了抗人肝细胞癌单克隆抗体HAb_(23) 。经过对51例肝癌,38例肝硬化,33例肝炎,36例正常组织,25例肝病组织及231例各种恶性肿瘤的免疫组化染色,肝癌的阳性率为82.3％,其它组织仅个别为阳性。免疫电镜定位显示,HAb_(23)主要定位于肝癌细胞的内质网等膜性结构。这一研究表明HAb_(23)与人体肝癌具有较好的特异性。

The human hepatocellular carcimona (HCC) cell line SMMG-7721 treated with human leucocytic α-interferon was used as an immunogen to prepare the monoclonal antibody against HCC. The anti-HCC monoclonal antibody (HAb23) was reacted with 51 cases of HCC, 38 cases of cirrhosis, 33 cases of hepatitis, 25 cases of other liver diseases, 36 cases of normal tissues including cryosections of fetus tissues and 231 cases of malignacies from various tissues by immunohistochemical staining. The results showed that 42 cases of HCC were positive. The positivit rate of HCC was 82.3%. Only 2 cases of liver cells around HCC. 1 case of cirrhosis and 4 cases of adenocarcinoma in gastrointestinal duct were weakly positi' ve. Others were negative. Immuno-electron microscopic localization showed that HAb23 was localized mainly on the surface membrane and on the cytoplasmic membranate structures of tumor cells. Our data suggest that the HAb23 is more relative specifity to HCC.