肺神经内分泌癌的免疫组化与免疫电镜研究

Studied on Neuroendocrine Carcinoma of the Lung by Immunohistochemistry and Immunoelectronmicroscopy

本文应用免疫组化与免疫电镜技术对20例肺神经内分泌癌进行了研究。6例非神经内分泌癌作为对照。结果表明:嗜铬颗粒蛋白A免疫组化染色19/20例阳性,免疫电镜能够比较特异地标记神经分泌颗粒,具有较大的确诊意义;神经特异性烯醇化酶染色20/20例为阳性,但非神经内分泌癌有3/6例阳性,特异性不强,适宜作为诊断的筛选指标;S-100蛋白可作为诊断的参考指标。

20 cases with neuroendocrine carcinoma of the lung (NECL) were studied by using immunohistochemistry and immunoelectronmicroscopy. 6 cases with non-NECL used as control of NEGL. Immunohistochemical staining for Chromogranin A (CgA) showed 19 positi-vity in 20 cases. Positive level were getting stronger from SCLC, AC to TC in parallel to the states of differentiation. Immunoelectronmicroscopy demonstrated same phenomena. CgA would play important role in pathological diagnosis. All 20 cases with NECL showed positive staining for NSE and 3 in the 6 cases with non-NECL were also positive. So we considered that NSE only could been used as a scanning marker.