核仁组成区相关蛋白在胃病变中的意义

Value of Nucleolar Organiser Region-Associated Protein (AgNOR) in Gastric Lesions

用银胶染色方法可以显示细胞核内的核仁组成区相关蛋白(AgNOR)。细胞核内AgNOR 计数可以揭示核仁的结构和功能奥秘。本文应用此法研究151例胃标本(其中50例胃炎,91例胃癌,10例正常胃组织)中的 AgNOR 均数,探讨其与病变发展,肿瘤分化、浸润转移及与预后的关系。结果显示正常胃组织,胃炎和胃癌细胞中 AgNOR 均数有显著差异,前者最少,后者最多。在胃癌中,发生浸润与转移的癌细胞中往往有较高的 AgNOR 计数,并且各型胃癌细胞核中 AgNOR 均数与患者预后密切相关,癌组织分化越差,AgNOR 均数越高,患者预后越差。癌细胞中 AgNOR 均数增高,反映癌细胞 RNA

Using a silver staining technique,nucleblar organiser regionassociated proteins(AgNORs)were studied in paraffin sections of 50 gastritis,91 gastric carcinoma and10 control cases.A significant difference in the mean number of AgNORs per nucl-eus was found when control and gastritis were compared with the malignant group.However,there was an overlap between each category.Further studies were warran-ted to determine that AgNORs counts could differentiate the two groups of infiltra-tion,metastasis with no infiltration,and gastric carcinoma without metastasis,al-though some overlap was observed.The number of AgNORs was related to the typesand prognosis of gastric carcinoma.The results showed that the poorer differentia-tion the tumours,had the higher AgNORs count per nucleus was and the poorerprognosis of the gastric carcinoma would be.It was suggested that AgNORs numbers refleted nuclear activity in particularrDNA transcription activity.