诺卡氏菌与假丝酵母的跨界融合及对退化养殖生态的修复

Inter-kingdom protoplast fusants between Nocardia sp. and Candida sp. used to bioremediation for the degeneration fishery ecology

运用溶菌酶和蜗牛酶破细胞壁 ,PEG和Ca2 + 促融的方法完成了诺卡氏菌和假丝酵母的跨界融合 ,得到具有亲本优势的融合细胞二株 :GW 0 1和GW0 2。通过菌落形态比较、抗生素抗性鉴定、耐热性和耐酸性比较、生长速率对比的分析 ,可确定其为真正的融合细胞。融合细胞较亲本细胞对水体和水质的适应范围更广 ,降解速率更快 ,能大幅去除养殖水体环境中的COD、NH+ 4 、NO-2 、PO3 -4等 ,稳定pH ,提高水中DO及水体生态环境中微生物和浮游生物的生物多样性 ,减少换水量 ,从而改善养殖生态环境 。

GW01 and GW02 were the fusants between Nocardia sp. and Candida sp. through inter kingdom protoplast fusion using PEG,Ca 2+ ,lysozyme and snailase. After analyzing the comparisons of form,resistance to antibiotics, heat resistance, acid resistance and the growth rate, GW01 and GW02 could be confirmed to be the true fusants. The fusants were more widely suitable to the fishery water quality than the parental strains. They could rapidly degrade the harmful composition of the water, such as COD,NH + 4,NO - 2,PO 3- 4, improve the concentration of DO and the biodiversity of the microorganisms in the water. The results suggested that the technique of inter kingdom protoplast fusion might offer ideally new strains for bioremediation for the degeneration fishery ecology.