摘要
目的 探讨尿脱落细胞CK2 0表达在膀胱癌诊断中的意义。 方法 应用免疫组化技术检测 4 0例膀胱癌患者和 2 0例非肿瘤患者尿脱落细胞CK2 0的表达。 结果 非肿瘤组尿脱落细胞无CK2 0表达 ,肿瘤组尿脱落细胞CK2 0表达率为 5 3% ,CK2 0与HE染色结合检测 ,阳性率达90 %。复发组尿脱落细胞CK2 0表达率 80 % ,明显高于初发组的 4 3% (P <0 .0 5 )。CK2 0表达率与肿瘤分级、分期无相关性。 结论 尿脱落细胞CK2 0表达检测特异性高 。
Objective To study the expression of cytokeratin(CK)20 in exfoliated urothelial cells and to evaluate its clinical significance. Methods The expression of CK20 in exfoliated urothelial cells in 40 patients with bladder cancer and 20 subjects with normal urothelium was observed with immunohistochemical methods. Results The positive staining of CK20 in patients with bladder cancer was 52.5%, the expression of CK20 was negative in all controls, the positive rate of expression of CK20 combining with HE staining in patients with bladder cancer was 90%. The expression of CK20 was significantly higher in recurrent cases(80%) than in primary cases(43.3%). CK20 was not related to the tumor grade or stage. Conclusions The results suggested that the method had a higher specificity and therefore CK20 would be a good diagnostic marker.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2002年第4期215-217,共3页
Chinese Journal of Urology