小麦春化发育相关蛋白质同工酶的研究

The Proteins and Isoenzymes Related to Vernalization of Wheat

本研究对不同发育特性的小麦品种 ,进行春化、脱春化处理 ,经凝胶电泳分析表明 ,在冬小麦春化过程中 ,不仅植株可溶性蛋白质含量增加 ,而且有新蛋白质的产生。英国Mercia品种与中国京 84 1都产生 5 3.2、4 6kD两种新的蛋白质 ,且在春化、脱春化中稳定存在 ,这两种蛋白质正是春小麦有而冬小麦未经春化没有的 ,表明这两种蛋白质的产生与冬性小麦春化过程密切相关 ;同时在春化过程中过氧化物酶 (POD)、酯酶 (EST)酶带及活性发生显著变化且产生了与特异蛋白质分子量 (5 3.2kD)接近的新带 ,表明POD和EST与小麦通过春化发育有一定相关性 ,而6 磷酸葡萄糖脱氢酶与春化发育相关性较小 ,超氧化物歧化酶 (SOD)

Three different wheat varieties in development properties were treated by means of vernalization, devernalization and analyzed by SDS PAGE. The results showed that not only the amount of soluble proteins increased, but also some new proteins appeared in the vernalization process of winter wheat. Two kinds of new proteins (53.2 kD, 46 kD) appeared at the same time in England Mercia and China Jing 841 and existed stably during the vernalization and devernalization process. These were also existed in spring wheat, but not in winter wheat before vernalization. This showed that newly synthesized proteins were highly related to vernalization. The molecular weight of the new bands of peroxidase and esterase isoenzymes in the vernalization process approached to that of the soluble protein. This result showed that peroxidase and esterase were highly related to vernalization of winter wheat. Glucose 6 phosphate isoenzyme was related to vernalization development in some degree. Yet, superoxide dismutase (SOD) isoenzyme was not related to vernalization development.