化学杀雄剂 1号对棉叶蛋白质氨基酸影响的研究

EFFECTS OF CHA NO.1 (A CHEMICAL GAMETOCIDE) ON THE CONTENTS OF AMINO ACIDS IN COTTON

利用化学杀雄剂 1号及其改良配方对棉花植株进行杀雄处理 ,研究结果表明 ,使用化学杀雄剂 1号后 ,棉叶 16种蛋白质氨基酸中Met,Ile ,Lue,His等 4种氨基酸的含量不受杀雄剂 1号的影响 ,而Pro ,Asp ,Glu ,Phe,Lys,Val,Tyr,Thr,Ser,Gly,Arg和Ala等 12种氨基酸含量受到不同程度的影响。化学杀雄剂 1号中添加脯氨酸以及添加混合氨基酸 (脯氨酸和谷氨酸 )的两种改良配方使不育株棉花棉叶Pro ,Asp含量恢复到接近对照水平 ,而对其他氨基酸含量则没有显著影响 ;其杀雄效率与单独喷施化学杀雄剂 1号无显著性差异 ,而毒副作用明显减轻。化学杀雄剂

CHA No.1, a gametocide developed by the researchers of Central Laboratory of Southwest Agricultural University, was used to spray the leaves of cotton plants, and amino acid contents in them were measured. The contents of Pro, Asp, Glu, Phe, Lys, Val, Tyr, Thr, Ser, Gly, Arg and Ala were affected, to different extents, by the foliar spray, while those of Met, Ile, Lue and His were not influenced. Compared with CHA No.1, the two modified preparations (CHA No.1 supplemented with proline or with a mixture of proline and glutamic acid) restored Pro and Asp to the levels close to those in the control, but had no effect on the other amino acids. The modified preparations showed a gametocidal effect similar to that of CHA No.1, while their toxic effect on the cotton plants was greatly alleviated. Disorder in amino acid metabolism induced by the gametocide is presumed to be responsible for pollen abortion in the cotton plants.