2-(3-羧基-2,4,5-三氮唑偶氮)-5-乙酰氨基苯酚的合成及其与钴的显色反应研究

Synthesis of 2-(3-carboxy-2,4,5-triazolylazo)-5-acetamidophenol and its color reaction with cobalt

合成了新试剂 2 -(3 -羧基 -2 ,4,5 -三氮唑偶氮 ) -5 -乙酰氨基苯酚 (CTZAAP) ,并研究了其与钴的显色反应及应用。在 p H7.61的 Na H2 PO4 -Na2 HPO4 缓冲液中该试剂与钴形成 2∶ 1红色稳定配合物 ,λmax为 5 2 9.2 nm,钴量在 0～ 1 .2 μg·m L- 1 范围内符合比尔定律 ,表观摩尔吸光系数为 4.61× 1 0 4 L· mol- 1 · cm- 1 。方法用于维生素 B1 2 注射液及环境水样中的微量钴测定 。

A reagent 2 (3 carboxy 2,4,5 triazolylazo) 5 acetamidophenol was synthesized and its color reaction with cobalt was studied The experiment results show that cobalt reacts with the reagent to form stable(1∶2) complex in the presence of NaH 2PO 4 Na 2HPO 4 at pH 7 61 The apparent molar absorptivity is 4 61×10 4L·mol -1 ·cm -1 at λ max =529 2 nm Beer′s law is obeyed in the range of 0~1 2 μg·mL -1 of cobalt The method has been applied to spetrophotometric determination of trace cobalt in VB 12 injection and environment water with satisfactory results