高效液相色谱法测定螺旋霉素发酵液中的有机酸

Determination of Organic Acids in Fermentation Broth of Spiramycin by High Performance Liquid Chromatography

采用高效液相色谱法在Zorbax 30 0 SBC18色谱柱 (5 μm ,4 6mmi.d .× 15cm)上以 0 0 1mol/L磷酸缓冲液(pH 2 32 )和甲醇的二元流动相梯度分离测定了螺旋霉素发酵液中的有机酸 ,流动相的流速为 0 6mL/min ,紫外检测波长为 2 10nm。实验结果表明 ,该方法的相对标准偏差为 0 33%～ 0 10 % ,回收率为 99 95 %～ 10 0 0 8%。方法简便、快速、可靠。

A method for determining organic acids in spiramycin fermentation broth by high performance liquid chromatography is described The operating conditions were Zorbax 300 SB C 18 column (5 μm,4 6 mm i.d.×15 cm) at 35 ℃, 0 01 mol/L phosphoric acid buffer solution (pH 2 32) and methanol as mobile phase at a flow rate of 0 6 mL/min and UV detection at 210 nm The relative standard deviations were 0 33%-0 10% and the recoveries were 99 95%-100 08% It's a simple, rapid and accurate method