毛细管电泳叠加对比法测定阿片粉中吗啡的含量

Quantitative Determination of Morphine in Opium Powder by Addition and Correlation Method Using Capillary Electrophoresis

用毛细管区带电泳 ,以叠加对比法对阿片粉中的吗啡进行了定量分析 ,样品的回收率为 10 0 .6 % ,色谱峰迁移时间的相对标准偏差 (RSD)不大于 2 .4 % ,相对迁移时间的RSD不大于 1.1% ,相对峰面积的RSD不大于 0 .5 1%。与内标对比工作曲线法作了比较 ,实验表明两种分析方法的结果无显著性差异 ,叠加对比法具有简便、快速、准确的优点 。

The morphine in opium powder has been quantitatively determined by addition and correlation method (ACM), in which capillary zone electrophoresis was applied,and the average recovery was 100.6% The relative standard deviation (RSD) of migration time was not more than 2 4%,the RSD of relative migration time was not more than 1.1%, and the RSD of the relative area was not more than 0.51%. Meanwhile, the contrast test has been done by the calibration curve method with an internal standard correlation The content of morphine in opium powder determined by ACM was the same as that by using the calibration curve method with an internal standard correlated The study shows that ACM is simple, quick and accurate