生物检材中乌头类生物碱的检验

Determination of Aconitine in Biological Samples

用薄层色谱 (TLC)、高效液相色谱 光电二极管阵列检测器 (HPLC/DAD)、动物实验等方法对生物检材中乌头生物碱进行检验 ,并对这些方法进行比较。结果发现 ,TLC法为此类药物检验筛选的首选方法 ,最小检出量为 0 3μg。在进行HPLC检测时 ,以乌头生物碱的特征紫外吸收光谱和动物实验结果为重要的定性手段 ,其特征吸收波长为 (2 2 8± 2 )nm和 (2 75± 2 )nm。乌头生物碱在 2mg/L～ 5 0mg/L时其色谱峰面积与质量浓度有很好的线性 ,相关系数为 0 9996。经实际案件证明 ,方法准确、灵敏 ,可用于生物检材中乌头类生物碱的检验。

The determination of aconitine in biological samples by using TLC, HPLC and animal tests is described TLC is simple, easy to manipulate and highly sensitive. The minimum detection limit was 0 3 μg, so it can be used as a preliminary screening method While detecting by HPLC, the characteristic UV absorption spectrum of aconitine and animal tests were used as the qualitative methods The characteristic UV absorption wavelengths were (228±2) nm and (275±2) nm. The linear range of calibration curve was 2 mg/L-50 mg/L The coefficient of variation was 0 999?6 It is suitable for applications in forensic field.