顶空固相微萃取-气相色谱法测定酒中的甲醇和杂醇油

Determination of Methanol and Fusel Oils in Alcohol Beverages Using Headspace Solid-Phase Microextraction and Gas Chromatography

采用环氧树脂作为固相涂层制作固相微萃取 (SPME)装置 ,建立了顶空固相微萃取 气相色谱法 (HS SPME GC)测定酒精饮料中甲醇和杂醇油的方法 ,并对萃取条件和色谱条件进行了优化。方法的检出限为 0 0 2mg/L～0 0 4mg/L ,相对标准偏差为 1 4 %～ 4 1% ;与顶空气相色谱法相比 ,灵敏度可提高 2 0倍～ 30 0倍。将该法用于啤酒、葡萄酒和保健酒中的甲醇和杂醇油的测定 ,加标回收率为 80 8%～ 110 3% ;与顶空气相色谱法 (国家标准方法 )进行了比较 ,相对误差不大于 7 3%。该法简便、快速、灵敏、精密度好 ,拓宽了SPME的应用范围。

A method for the determination of methanol and fusel oils in alcohol beverages using headspace solid phase microextraction and gas chromatography (HS SPME GC) is presented The solid phase was a coated epoxy resin. The extraction and chromatography conditions were optimized Limits of detection were 0 02 mg/L-0 04 mg/L and relative standard deviations were in the range of 1 4%-4 1%. The proposed method showed better sensitivity in comparing with direct headspace gas chromatography(HS GC,the National Standard method) This method was applied to evaluate real samples The spiked recoveries in beer, wine and functional alcohol samples ranged from 80 8% to 110 3% for methanol and fusel oils The results by HS SPME GC and HS GC for alcohol samples coincided very well The proposed method is simple, fast and accurate with high reproducibility, high sensitivity and low cost It extends the applications of SPME