荧光共焦显微术与多光子显微术的差别

Confocal Fluorescence Microscopy and Multiphoton Microscopy

共焦显微术和多光子显微术因其可以对厚的生物样品实现光学断层成像 ,因而在生物医学等领域具有广泛的应用前景。本文详细综述了共焦显微术和多光子显微术在成像原理及特性等方面的差别。在此基础上 ,讨论了每种显微术各自的优点。

This paper reviews the differences between the confocal fluorescence microscopy and multiphoton microscopy in imaging principle and image properties.The advantages,diadvantages and applications are also discussed in detail for every technique,respectively.