基因组DNA的提取及RAPD条件优化

Isolation of genomic DNA and optimization of RAPD conditions of nai

提取了木奈基因组 DNA,并以其为模板对影响 RAPD扩增的重要参数进行优化试验 ,以期建立木奈 RAPD反应的优化体系 .结果表明 ,PCR扩增体系最佳条件是 :2 0 μL体积中 ,2 0 0 μmol·L- 1 d NTP、2 .5 mmol· L- 1 Mg Cl2 、0 .2 μmol· L- 1随机引物、2 5 .0 ng·μL- 1 DNA模板、1 -2 U

The method for isolating genomic DNA from nai ( Prunus salicina Lindl. var. cordata J.Y.Zhang et al.) was studied. Based on the genomic DNA, some essential factors that might affect the results of RAPD were compared and the optimal RAPD reaction system for nai was established. A 20 μL solution with 2.5 mmol·L -1 MgCl 2 , 200 μmol·L -1 dNTP, 0.2 μmol·L -1 random primer, 25.0 ng·μL -1 genomic DNA, 1-2 U Tag DNA polymerase would be bese for amplification reaction.