胰岛素样生长因子系统在人早孕绒毛滋养层细胞中的表达

Gene Expression of Insulin-Like Growth Factors in Human Villous Trophoblast Cells of Early Pregnancy in Vitro

目的 探讨胰岛素样生长因子系统 (IGFs)在体外培养的人早孕绒毛滋养层细胞中的基因表达及其作用。方法 采用逆转录 -聚合酶链反应 (RT- PCR)技术半定量检测体外培养的人早孕绒毛滋养层细胞中IGFs m RNA的表达。结果 体外培养的人早孕绒毛滋养层细胞中可以检测出 IGF- m RNA、IGF- R m RNA、IGFBP- 3m RNA的表达。结论 IGFs可能在早期细胞滋养层的侵入、增殖分化、胎盘形成和胚胎生长发育过程中以自分泌和

Objective To investigate the gene expression of insulin-like growth factors(IGFs) in human villous trophoblast cells of early pregnancy in vitro and their action. Methods Semiquantitative reverse transcriptase polymerase chain reaction(RT-PCR), with β-ACTIN as internal standard, was applied to determine the expression of IGFs messenger RNA in human villous trophoblast cells of early pregnancy in vitro. Results The expression of IGF-Ⅱ mRNA,IGF-ⅠR mRNA and IGFBP-3 mRNA was detectable in human villous trophoblast cells of early pregnancy in vitro. Conclusion IGFs appear to play important regulation roles in early invasion, proliferation and differentiation of cytotrophoblast, and in the formation of placenta and the development of embryo via autocrine and/or paracrine way.