对氨基苯甲酸对粘性放线菌生长影响的研究

Effect of Para-aminobenzoic Acid on the Growth of Actinomyces viscosus

目的 探讨对氨基苯甲酸 (PABA)对牙根面龋致病菌之一的粘性放线菌生长的影响。方法 在加入不同浓度 PABA的改良 Carlsson培养基中采用厌氧培养技术 (80 % N2 ,10 % H2 ,10 % CO2 )培养细菌 48小时 ,通过 UV- 16 0 1紫外可见分光光度仪测定细菌浓度 OD值 (λ=5 40 nm) ;并用平板倾注法对细菌进行计数及琼脂平板培养观察细菌生长的情况。结果 10 - 1 0～ 10 - 4 g/ L 浓度的 PABA有促进粘性放线菌生长的能力 ,且当 PABA浓度增加到 10 - 6g/ L 时 ,促进生长的作用达最强 ;当 PABA浓度增加到 10 - 3 g/ L 时 ,对粘性放线菌的生长无明显的促进作用。结论 PABA可促进粘性放线菌的生长 ,在 PABA浓度为 10 - 6g/ L 时 。

Objective To examine the effects of para-aminobenzoic acid (PABA) on the growth of Actinomyces viscosus. Methods Different concentrations of PABA (10 -10-10 -3g/L) were each transferred to modified Carlsson medium. Actinomyces viscosus ATCC19246 grew in them. And the cultures were incubated at 37℃ anaerobically in the atmosphere of 80%N 2, 10%H 2, 10%CO 2 for 48h. Actinomyces viscosus OD values (λ=540nm) were obtained with UV-1601. Colony forming unit (CFU) was established by growth of Actinomyces viscosus in culture when different concentrations of PABA (10 -10-10 -3g/L) were present. Results Different concentrations of PABA (10 -10-10 -4g/L) had different stimulating effects on the growth of Actinomyces viscosus (P<0.05). But this kind of stimulating effect declined when PABA concentration was 10 -5g/L, and as PABA (10 -3g/L) was present, this kind of effect was missing. Conclusion The phenomena indicated that PABA has stimulating effect on the growth of Actinomyces viscosus, particularly when PABA is at the concentration of 10 -6g/L.