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对氨基苯甲酸对粘性放线菌生长影响的研究 被引量:1

Effect of Para-aminobenzoic Acid on the Growth of Actinomyces viscosus
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摘要 目的 探讨对氨基苯甲酸 (PABA)对牙根面龋致病菌之一的粘性放线菌生长的影响。方法 在加入不同浓度 PABA的改良 Carlsson培养基中采用厌氧培养技术 (80 % N2 ,10 % H2 ,10 % CO2 )培养细菌 48小时 ,通过 UV- 16 0 1紫外可见分光光度仪测定细菌浓度 OD值 (λ=5 40 nm) ;并用平板倾注法对细菌进行计数及琼脂平板培养观察细菌生长的情况。结果  10 - 1 0~ 10 - 4 g/ L 浓度的 PABA有促进粘性放线菌生长的能力 ,且当 PABA浓度增加到 10 - 6g/ L 时 ,促进生长的作用达最强 ;当 PABA浓度增加到 10 - 3 g/ L 时 ,对粘性放线菌的生长无明显的促进作用。结论  PABA可促进粘性放线菌的生长 ,在 PABA浓度为 10 - 6g/ L 时 。 Objective To examine the effects of para-aminobenzoic acid (PABA) on the growth of Actinomyces viscosus. Methods Different concentrations of PABA (10 -10-10 -3g/L) were each transferred to modified Carlsson medium. Actinomyces viscosus ATCC19246 grew in them. And the cultures were incubated at 37℃ anaerobically in the atmosphere of 80%N 2, 10%H 2, 10%CO 2 for 48h. Actinomyces viscosus OD values (λ=540nm) were obtained with UV-1601. Colony forming unit (CFU) was established by growth of Actinomyces viscosus in culture when different concentrations of PABA (10 -10-10 -3g/L) were present. Results Different concentrations of PABA (10 -10-10 -4g/L) had different stimulating effects on the growth of Actinomyces viscosus (P<0.05). But this kind of stimulating effect declined when PABA concentration was 10 -5g/L, and as PABA (10 -3g/L) was present, this kind of effect was missing. Conclusion The phenomena indicated that PABA has stimulating effect on the growth of Actinomyces viscosus, particularly when PABA is at the concentration of 10 -6g/L.
出处 《华西医科大学学报》 CAS CSCD 北大核心 2002年第2期210-211,219,共3页 Journal of West China University of Medical Sciences
基金 国家自然科学基金资助 (批准号 396 70 785 )
关键词 对氨基苯甲酸 粘性放线菌 影响 研究 微生态 细菌生长 PABA Actinomyces viscosus Para-aminobenzoic acid Microecology Microbial growth
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  • 1别列卓夫斯基BM 龙琨(译).微生物化学[M].北京:中国工业出版社,1965.240.
  • 2李季伦 张伟心 等.微生态生理学[M].北京:北京农业大学出版社,1993.76-80.
  • 3胡涛,华西口腔医学杂志,1998年,16卷,146页
  • 4李季伦,微生物生理学,1993年,76页
  • 5李季伦,微生态生理学,1993年,76页
  • 6龙琨(译),微生物化学,1965年,240页
  • 7胡涛,周学东,张萍,肖晓蓉,刘豫蓉.血液链球菌产生对氨基苯甲酸的实验研究 Ⅱ.对氨基苯甲酸的定性定量分析[J].华西口腔医学杂志,1998,16(2):149-152. 被引量:4

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  • 1Rosenberg M, Gumtnick D, Rosenberg E. Adherence of bacteria to hydrocarbons: A simple method for measuring cell-surface hydrophobicity. FEMS Microbiol Letters, 1980 ; 9 (1) ; 29.
  • 2Rosenberg M. Basic and applied aspects of microbial adhesion at the hydrocarbon: water interface. Crit Rev Microbiol, 1991, 18(2):159.
  • 3Dill KA. The meaning of hydrophobicity. Science, 1990; 250(4978):297.
  • 4William BC, Marcia DL, Beem JE, et at. Relative hydrophobicities of Actinomyces viscosus and Actinomyces naeslundii strains, and their adsorption to saliva-treated hydroxyapatite. Infect Immun, 1985; 47(3): 730.
  • 5Carlsson J. Nutritional requirements of streptococcus mutans and streptococcus sanguis in mixed culture. Arch Oral Biol,1971; 16(8): 963.
  • 6郭斌 周学东 肖晓蓉等.共生因子PABA实验基础培养基的筛选[J].华西口腔医学杂志,2000,18(1):23-23.

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