多粘菌素B对大鼠内毒素血症血浆中内毒素和肿瘤坏死因子-α水平的影响

Effect of polymyxin B on levels of endotoxin and tumor necrosis factor-α in plasma of endotoxaemia in rats

目的 :观察多粘菌素B(PMX B)对大鼠内毒素血症血浆中内毒素 (LPS)和肿瘤坏死因子 α(TNF α)水平的影响。方法 :实验分为 :LPS +PMX B组 ;LPS +生理盐水 (NS)组 ;LPS模型组。给大鼠一次LPS 0 5mg·kg-1,iv ,前 2组分别于注入LPS后 0 5h给予PMX B 0 2mg·kg-1和 1mLNS ,iv ,LPS模型组大鼠于注入LPS后不作其他处理。在给LPS后 0 5 ,1,2 ,4h抽血 ,用鲎试剂检测血浆中LPS ,ELISA法测定TNF α水平。结果 :LPS +PMX B组 ,血浆LPS ,TNF α水平明显下降 ,平均动脉压 (MAP)于 2 ,4h明显高于LPS +NS组和LPS模型组。结论 :PML B治疗能明显降低血浆中LPS ,TNF α水平。

AIM: To evaluate the effect of polymyxin B on endotoxin and tumor necrosis factor α (TNF α) in plasma of endotoxaemia in rats. METHODS: Wistar rats were divided into 3 groups randomly: the group of lipopolysaccharide (LPS)+polymyxin B were treated with polymyxin B sulfate 1 mL (0 2 mg·kg -1 ) in 30 min after giving endotoxin, the group of LPS+normal saline (NS) were treated with NS solution 1 mL 30 min after giving endotoxin, the group of LPS model were treated without anything. Quantitative endotoxin determination in blood with chromogenic modification of the limulus amebocyte lysate test, tumor necrosis factor α (TNF α) of the blood were detected by ELISA. RESULTS: After treated with polymyxin B, the concentration of LPS and TNF α of plasma were significantly decreased. CONCLUSION: Polymyxin B can be used to reduce levels of TNF α and LPS in blood.