健脾理气中药上调nm23表达

Jian Pi Li Qi Herbs(JH) up-regulate nm23

目的 :观察健脾理气中药对肿瘤肝转移灶nm2 3基因表达的影响。方法 :采用W2 56 癌肉瘤细胞系脾下极接种造模。中药组动物灌喂健脾理气中药 0 .3ml(浓度相当于生药剂量 2g ml) ,每日一次 ;对照组则灌喂等体积生理盐水 ,连续给药 2 1天后处死动物 ,剥离脾脏瘤结节 ;计数肝脏转移结节数 ,分离瘤组织 ,以原位杂交法检测瘤组织中nm2 3mRNA的表达。结果 :健脾理气中药明显抑制脾脏移植瘤生长 ,肝转移结节数平均 7.3± 2 .2 6个 ,转移抑制率为 4 1.6 % ;中药组nm2 3阳性表达率高达 80 % ,且有 30 %的样本呈强阳性表达 ,与对照组相比差异显著 (P <0 .0 5 )。结论 :健脾理气中药可上调nm2

Purpose:To ascertain JH's effects on nm23 expression in liver metastases. Methods:To replicate liver metastasis model, W 256 carcinosarcoma cells were injected into lower part of Wistar rats. At the day of modeling, all rats were randomly divided into JH group and control group. JH group were administrated orally 0.3 ml JH once a day for 21 days; control group were given equal volumes of natural saline in the same way. On the day after experimental therapy was completed, animals were killed, incised tumor nodules, counted the number of metastatic clones on liver, and nm23mRNA were detected by hybridization in situ. Results:JH can markedly inhibit transplanted tumor growth in spleen. As there were only 7.3 2.26 metastatic clones in the JH group, its metastasis inhibitory rate reached as high as 41.6%. The results from hybridization in situ of metastasis tumor tissues showed that the nm23 expression rate in the JH group was 80%, which were significantly higher than that of the control group( P <0.05). Conclusions:JH can up regulate nm23 expression in liver metastases.