MAGE—4基因在肝细胞肝癌中的表达及临床意义

Expression and clinical significance of MAGE-4 gene in human hepatocellular carcinoma

目的 通过研究ＭＡＧＥ—４基因在肝细胞肝癌组织中的表达，并与患者临床资料进行分析，探讨ＭＡＧＥ—４基因与肝细胞肝癌（ＨＣＣ）患者临床指标及转移与复发的关系，为ＭＡＧＥ—４基因编码蛋白用于ＨＣＣ患者免疫治疗提供依据。方法 用ＲＴ—ＰＣＲ的方法对３１例ＨＣＣ患者癌组织及相应癌旁组织ＭＡＧＥ—４基因表达进行测定，对全部ＲＴ—ＰＣＲ扩增产物中目的基因片段进行ＤＮＡ测序以证实其为ＭＡＧＥ—４基因，患者均测定并统计ＡＦＰ、ＡＦＵ、抗ＨＣＶ、ＨＢｓＡｇ、ＡＦＰ ｍＲＮＡ、肿瘤直径等临床指标。结果 ３１例ＨＣＣ患者肝癌组织中ＭＡＧＥ－４基因表达的阳性率 ３８．７％（１２／３１）明显高于癌旁组织中 ＭＡＧＥ—４基因表达的阳性率０％（０／３１），Ｐ＜０．０１。ＨＣＣ患者肝癌组织中ＭＡＧＥ—４基因表达的阳性率与患者ＡＦＰ、ＡＦＵ、抗ＨＣＶ、ＨＢＶ标志物、ＡＦＰ ｍＲＮＡ、肿瘤直径等临床指标均无关，Ｐ＞０．０５。结论 ＭＡＧＥ－４基因在ＨＣＣ患者肝癌组织中特异高表达，可能作为ＨＣＣ患者免疫治疗攻击的靶点，ＨＣＣ患者肝癌组织中ＭＡＧＥ—４基因表达的阳性率与ＨＣＣ患者肿瘤标忘物、转移、复发均无关。

Objective To explore the possibility of MAGE-4 gene encoding protein used as a target for immuno- therapy in HCC patients. Methods The expression of MAGE-4 gene in tumor tissues and tumor adjacent non-HCC liver tissues was examined by the RT-PCR method. The relationship between positive expression rate of MAGE-4 gene and other clinical and lab data including AFP, AFU, anti-HCV, HBsAg, AFP mRNA, and the diameter of the tumors in HCC patients was also determined. Results The positive expression rate of MAGE-4 gene was significantly higher in the tumor than in tumor surrounding tissues (38.7% vs 0%, P<0.01), while the positive expression rate of MAGE-4 gene had no relationship with the clinical and lab data (P>0.05). Conclusions The high frequency of MAGE-4 gene expression in HCC suggests the possibility of MAGE-4 gene encoding protein as a target for immunotherapy in HCC patients, but the expression has no relationship with the tumor metastasis and the recurrence of HCC.