血管内皮生长因子和基质金属蛋白酶MMP-2、MMP-9在子宫颈癌中表达的免疫组化研究

Immunohistochemical study of vascular endothelial growth factor, matrix metalloproteinase-2 and matrix metallopro-teinase-9 inhuman cervical carcinoma

目的 研究血管内皮生长因子（ＶＥＧＦ）与基质金属蛋白酶ＭＭＰ－２和ＭＭＰ－９在子宫颈癌组织中的表达与子宫颈癌的生长、浸润及转移的关系。方法 采用免疫组织化学方法对５７例子宫颈癌（其中子宫颈鳞癌３０例、子宫颈腺癌２０例、子宫颈腺鳞癌７例），２９例宫颈上皮内瘤样病变（ＣＩＮ）和１６例正常子宫颈组织进行标记及分析。结果 随着宫颈病变的进展，ＶＥＧＦ、ＭＭＰ－２、ＭＭＰ－９的阳性表达率逐渐增高。三者在正常宫颈组织、ＣＩＮ、子宫颈鳞癌、腺癌、腺鳞癌的细胞浆中的阳性表达率分别为：ＶＥＧＦ的阳性表达率为：３／１６（１．８％）、２４／２９（８．８％）、２８／３０（９３．３％）、２０／２０（１００％）、７／７（１００％）。ＭＭＰ－２的阳性表达率为：４／１６（２５％）、１３／２９（４４．８％）、２３／３０（７６．７％）、１７／２０（８５％）、７／７（１００％）。ＭＭＰ－９的阳性表达率为：４／１６（２５％）、１８／２９（６２．１％）、１８／３０（６０．０％）、１９／２０（９５％）、７／７（１００％）。ＭＭＰ－２在各种宫颈组织的间质细胞中也均为阳性表达，而 ＭＭＰ－９在正常宫颈组织和 ＣＩＮ级的间质中无阳性表达，在ＣＩＮⅡ级和各宫?

Objective To study the relationship of the expression of Vascular Endothelial Growth Factor (VEGF), Matrix Metalloproteinase - 2( M MP - 2)and Matrix Metalloproteinase - 9( M MP - 9)in the development, invasion and metastasis of human cervical carcinomas. Methods VEGF, MMP -2 and M MP -9 were stained immunohistochemically in 57 cases of cervical carcinoma( 30 cases of squamous - cell carcinoma of the uterin cervix ,20 cases of adenocarcinoma of the uterin cervix, 7 cases of glandular and squamou - cell carcinoma of the uterin cervix) ,in 29 cases of cervical intraepithelial neoplasia( CI N) and 16 cases of normal cervices. Results The expression rates of VEGF, MMP - 2 and MMP - 9 were gradually increased with the progress cervical lesions. The positive rates of VEGF,MMP- 2 and MMP- 9 in normal cervical epithelium, CIN, squamous - cell carcinoma, adenocarcinoma, glandular and squamous cell carcinoma were 3/16(18.8%) ,24/29(82.8%), 28/30(93.3%),20/20(100%),7/7(100%); 4/16(25%),13/29(44.8%),23/30(76.7%),17/20(85%),7/7(100%); and 4/16(25%), 18/29(62.1%), 18/30(60.0%), 19/20(95%),7/7(l00%),respectively. Peritunoral staining stromal cells for MMP- 2 was observed in all cases. Pertumaral staining of stromal cells for MMP -9 was observed in carcinomas, adenocarcinoma, glandular and squamous cell carcinoma, CIN II ,but was uniformly absent from the CIN I and from normal cervices. The positive rates of VEGF and MMP -9 in invasive cervical carcinoma groups and CIN group were obviously higher than that in normal cervical epithelium group ( P < 0.01) . The positive rates of M MP - 2 in invasive cervical carcioma groups were obviously higher than that in normal cervical epithelium group( P <0.05) . No significant correlation could be established among the expression of VEGF, MMP - 2 and MMP - 9 and the clinical stage, tumor diameter, pathologic grade and pelvic node metastasis. There were positively correlations among VEGF, MMP - 2 and MMP - 9.Conclusions The expression of VEGF, MMP -2 and MMP -9 were significant correlation in different cervical lesions, and they play a cooperated role in the angiogenesis and invasion of cervical carcioma.