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等电聚焦电泳/免疫固定和考马氏蓝染色技术检测缺糖转铁蛋白 被引量:2

Isoelectric focusing/immunofixation/Comassie blue staining for detection of carbohydrate-deficient transferrin
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摘要 目的 建立等电聚焦电泳/免疫固定(IEF/IF)和考马氏蓝染色技术检测缺糖转铁蛋白(CDT)方法。方法选择32例酒精性肝病患者,血清铁饱和后,进行水平板式超薄层等电聚焦电泳,免疫固定和考马氏蓝染色后,蛋白条带进行等电点分析。结果 酒精性肝病组CDT阳性率为87.5%。CDT诊断酒精性肝病的灵敏度为87.5%,特异度为69.39%。结论IEF/IF结合考马氏蓝染色技术是一种操作方便、经济、灵敏度高、分辨率高、适用于进一步大样本检测血清CDT的方法。 Objective To establish the detect method of Carbohydrate - deficient Transferrin. Methods According to the-diagnosis criteria for alcoholic liver disease established by Chinese Medical Association in NanJing 1995,32 alcoholic liverdisease patients were selected. Each serum sample was iron saturated with l0mmol/L Fe solution giving a 1:4 find dilutionat 37d. Different molecular forms of transferrin were identified by isoelectric focusing on polyacrylamide gel, pI5- 7. Prepared samples were applied after prefocusing of the gel for 500Vh (l800V,20mA,15W),the samples were applied to the gel for 1 hour (1800V,2OmA,15W),and isoelectric separation was done by 1800V,20mA,15W and 10°C .After isoelectric focusing, draw a diagram of ph - cm according to the ph value of gel from anode to cathode. Immunofixation was employed to identify transferrin. The gel were incubated at 37°C for 2 hours with anti - human transferrin serum. The unprecipitated protein were washed with 10% NaCl, 0.9% NaCl and distilled water. Comassie blue staining were used to show the protein zone. Carbohydrate - deficient Transferrin were defines as had transferrin band at pI≥ 5.7. Results The positive ratio of Carbohydrate - deficient Transferrin was to be raised in alcoholic liver disease (87.5%) . Conclusion Carbohydrate - deficient transferrin can be detected by isoelectric focusing / immunofixation / Comassie blue stailning.
出处 《中国实验诊断学》 2002年第2期101-103,共3页 Chinese Journal of Laboratory Diagnosis
关键词 等电聚集电泳/免疫固定 考马氏蓝染色技术 缺糖转铁蛋白 酒精性肝病 CDT isoelectric focusing immun of ixation alcohoic liver disease
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  • 1袁平戈 周卫平.酒精性肝病及肝纤维化学术研讨会纪要[J].肝脏病杂志,1995,3:196-196.
  • 2郭尧君.薄层等电聚焦技术[J].生物物理学报,1998,4(4):389-389.

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  • 1鲁彦,高公民,李依萍,李德红.缺糖转铁蛋白的检测在慢性酒精中毒诊断中的作用[J].检验医学,2007,22(1):91-93. 被引量:5
  • 2Lott JA, Curtis LW, Thompson A. Reported alcohol consumption and the serum carbohydrate-deficient transferrin test in third-year medical students. Clin Chim Acta, 1998,276:129-141.
  • 3Sillanaukee P, Olsson U. Improved diagnostic classification of alcohol abusers by combining carbohydrate-deficient transferrin and gamma-glutamyltransferase. Clin Chem, 2001,47:681-685.
  • 4Golka K, Sondennann R, Reich SE. Carbohydrate-deficient transferrin (CDT) as a biomarker in persons suspected of alcohol abuse. Toxicol Lett, 2004,151:235-241.
  • 5Lothar Thomas.Clinical Laboratory Diagnostics.临床实验诊断学.朱汉民,沈霞,吕元,等,译.上海:上海科学技术出版社,2004:633-636.
  • 6Arteel G, Marsano L, Mendez C, et al. Advances in alcoholic liver disease. Best Pract Res Clin Gastroenterol, 2003,17:625- 647.
  • 7Miller PM, Anton RF. Biochemical alcohol screening in primary health care. Addict Behav, 2004,29 : 1427-1437.
  • 8Chen CH,Walker J,Momenan R. Relationship between liver function and brain shrinkage in patients with alcohol dependence[J].Alcoholism:Clinical and Experimental Research,2011.
  • 9Cylwik B,Daniluk M,Chrostek L. Effect of body iron stores in the indicators of alcohol abuse and alcoholic liver injury[J].Pol Merkuriusz Lek,2010,(168):450-453.
  • 10Cylwik B,Chrostek L,Daniluk M. The assessment of serum soluble transferrin receptor in alcoholics[J].Clinical and Experimental Medicine,2010,(01):73-79.

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