^(99m)Tc-MIBI评价川芎嗪逆转乳腺癌MCF-7/ADR细胞耐多药的`研究

Evaluation of Reversal of Multidrug Resistance of MCF-7/ADR Cell line by Tetramethylpyrazine with99m Tc-MIBI

目的:用P-gp的底物^(99m)Tc-MIBI评价中药钙通道阻滞剂川芎嗪(TMP)对乳腺癌MCF-7/ADR细胞耐多药的逆转作用。方法:以经典逆转剂维拉帕米和MCF-7/WT细胞作对照,各组均加入^(99m)Tc-MIBI,分别测定逆转剂作用前后细胞内(C_(in))和上清液(C_(out))中的放射性活度的比值。结果:在加入逆转剂之前MCF-7/WT和MCF-7/ADR细胞株间^(99m)Tc-MIBI聚集的差别为33倍,加入逆转剂 60min后二者间的差别为3.8倍。结论:^(99m)Tc-MIBI的变化可以反映耐多药的逆转,TMP可部分逆转MCF-7/ADR的耐多药性。

Objective:To evaluate the reversal effect of MDR of MCF-7/ADR cell by TMP with 99m Tc-MIBI. Method: The classical modulator verapamil and MCF-7/WT were served as the contrasts. 99m Tc-MIBI was added in each groups, the accumulation of 99m Tc-MIBI inside the cell to that outside the cell (Cin/Cout)was measured. Results: The WT/ADR differentials for 99mTc-MIBI accumulation at 60 min were 33-fold without the modulator and 3. 8-fold in the presence of 300 mg/L TMP. Conclusion: 99mTc-MIBI can represent the reversal of MDR, the MDR of MCF-7/ADR cell can be modulated by TMP.