经腹腔转染iNOS基因对大鼠的降血压作用

Effect of iNOS gene transfection via enterocoelia on blood pressure in rats

目的 :研究经腹腔转染人iNOScDNA进入正常大鼠体内对大鼠血压的影响及转染的iNOS基因在其体内表达的情况。方法 :经腹腔基因转染。结果 :①经腹腔转染iNOS基因大鼠的血压从转染后第 2d起明显较注射前降低 ,至第 6d降低仍有显著意义 (P <0 0 1) ;②转染iNOS基因大鼠在转染后第 3d、第 6d腹腔巨噬细胞的NO水平明显高于对照组 (P <0 0 1)。结论 :经腹腔转染iNOS能明显降低大鼠血压。

AIM: To investigate the effect of human iNOS gene transfection on blood pressure in SD rats. METHODS: Human iNOS cDNA was transfected using gene transfection technique via enterocolia. The iNOS gene expression in rat celiac macrophages was also observed. RESULTS: The significant lowering of blood pressure in rats transfected with iNOS gene was observed from second day to sixth day after transfection, compaered to the control group ( P<0.01 ). The NO level in transfected rat celiac macrophages at sixth day after transfection was singnificantly higher than that in control group.CONCLUSION: The results indicated that method of gene transfer via enterocolia may be useful for the hypertension therapy.