T淋巴细胞核仁组成区嗜银蛋白在肿瘤诊断中的应用

Clinical application of argyrophilic NOR associated proteins of T lymphocytes in diagnosis of cancer.

目的探讨外周血T淋巴细胞核仁组成区(NOR)嗜银蛋白的含量在肿瘤诊断中的应用价值。方法应用银染技术对体外激活的人外周血T淋巴细胞染色,通过计算机显微图象分析计算硝酸银染色的嗜银蛋白(AgNORs)面积与核面积的比值(I.S%),对58例健康人、69例良性病变和123例种瘤病人进行对比研究。结果健康人、良性病变和恶性肿瘤病人的T淋巴细胞AgNORs检测的I.S%均值分别为7.66±0.92%、7.52±0.91%和5.42±0.88%,其中肿瘤病人与健康者和良性疾病患者之间的T细胞AgNORs活性差异有显著意义(P<0.01),而健康人与良性疾病患者之间差异无显著性(P>0.05);不同肿瘤之间相比,T细胞AgNORs差异也无显著意义(P>0.05)。结论人外周血T淋巴细胞AgNORs的检测对肿瘤的临床诊断与应用具有重要的指导意义。

Objective To approach into the diagnostic value of cancer by deter-mining the argyrophilic NOR associated proteins in T Lymphocytes of different patients.Methods KL-2imaging system was used to study the areas of AgNORs in T cells.T cells from peripheral blood were cultivated and stimulated,and silver-staining was per-formed.Re sults The values of AgNORs of T lymphocytes from58normal adults,69patients with benign lesions and123patients with malignant neoplasms were studied.Their average I.S%values were7.66±0.92%?7.52±0.91%and5.42±0.88%respectively.The mean area of AgNORs in malignant group was lower than that of normal and benign groups(P<0.01),and no difference between benign and normal groups.In addition,the results showed that different malignant neoplamas were not significant differences.Con-clusion AgNORs of T lymphocytes in peripheral blood might serve as a new marker for the diagnosis of cancers.It is of great clinical significance in the diagnosis of cancers.