期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

改进和创新神经药理学研究方法 被引量：1

Improving and developing neuropharmacological research methods
下载PDF
导出
摘要 神经药理学面临创新性药物研究的任务 ,系统地改进和创新各种方法 ,应当引起高度重视。在靶基因寻找和实用化研究基础上 ,建立以细胞为基础的高通量筛选方法 ;对筛选出的先导化合物 ,在不同水平的实验模型上 ,建立以计算机技术为主的各种客观定量研究方法。
作者 魏尔清
机构地区 浙江大学医学院
出处 《浙江大学学报（医学版）》 CAS CSCD 2002年第2期65-67,共3页 Journal of Zhejiang University(Medical Sciences)
基金 国家自然科学基金项目 (G19990 5 4 0 0 3 39770 2 70 )
关键词 药物筛选 神经药理学 基因组 高通量筛选 计算机辅助技术
分类号 R96 [医药卫生—药理学]
  • 相关文献

参考文献13

  • 1魏尔清,沈建中.后基因组时代药理学研究趋向[J].生理科学进展,2002,33(1):7-11. 被引量：2
  • 2Pickar D, Rubinow K. Pharmacogenomics of psychiatric disorders[J]. Trends Pharmacol Sci,2001,22(1):75-83.
  • 3Taylor D L, Woo E S, Giuliano K A. Real-time mole-cular and cellular analysis: the new frontier of drug discovery[J]. Curr Opin Biotechnol,2001,12(1):75-81.
  • 4张纬萍,魏尔清,朱朝阳,梅如焕,赵孟辉.大鼠局灶性脑缺血模型的定量分析[J].浙江大学学报（医学版）,2002,31(2):72-76. 被引量：15
  • 5YU Yue-ping,XU Qiu-qin,ZHENG Ming-zhi,et al(俞月萍,徐秋琴,郑鸣之,等). Light transmission measurement of focal ischemic cerebral infarction in mice[J]. Journal of Zhejiang University (Medical Sciences) [浙江大学学报(医学版)],2002,31(2):91-93.(in Chinese)
  • 6YAN Le-qin,WEI Er-qing,HU Hai-tao,et al(严乐勤,魏尔清,胡海涛,等). Novel quantitative method for evaluating oxygen/glucose deprivation-induced injury of hippocampal slices[J]. Journal of Zhejiang University (Medical Sciences) [浙江大学学报(医学版)],2002, 31(2):81-85.(in Chinese)
  • 7YU Guo-liang,WEI Er-qing,HE Wei(余国良,魏尔清,何巍). A quantitative method for evaluating brain injury and neuroprotective drugs in brain slices of mice[J]. Journal of Zhejiang University (Medical Sciences) [浙江大学学报(医学版)],2002, 31(2):86-90.(in Chinese)
  • 8WANG Meng-ling,WEI Er-qing,YANG Xiao-hong,et al(王梦令,魏尔清,杨晓红,等). Optical recording method for evaluation of neuronal damage in rat hippocampal slices during ischemia and reperfusion[J]. Journal of Zhejiang University (Medical Sciences) [浙江大学学报(医学版)],2002, 31(2):94-97.(in Chinese)
  • 9XU A-jing,CHEN Zhong,LI Ren,et al(徐阿晶,陈忠,李韧,等). Effects of TAK-147, a novel acetylcholinesterase inhibitor, on spatial memory deficits as evaluated by Morris water maze of rats[J]. Journal of Zhejiang University (Medical Sciences) [浙江大学学报(医学版)],2002, 31(2):98-102.(in Chinese)
  • 10ZHANG Li-hui,WEI Er-qing(张丽慧,魏尔清). Time course of brain damage and expression of related molecules in the rats with transient global cerebral ischemia[J]. Journal of Zhejiang University (Medical Sciences) [浙江大学学报(医学版)],2002, 31(2):77-80.(in Chinese)

二级参考文献45

  • 1[1]Pickar D, Rubinow K. Pharmacogenomics of psychiatric disorders. Trends Pharmacol Sci,2001,22 75～83.
  • 2[2]Cunningham MJ. Genomics and proteomics: The new millennium of drug discovery and development. J Pharmacol Toxicol Methods,2000,44 291～300.
  • 3[3]Walter G, Bussow K, Cahill D, et al. Protein arrays for gene expression and molecular interaction screening. Curr Opin Microbiol,2000,3298～302.
  • 4[4]Netzer R, Ebneth A, Bischoff U, et al. Screening lead compounds for QT interval prolongation. Drug Discov Today,2001,6 78～84.
  • 5[5]Hertzberg RP, Pope AJ. High-throughput screening: new technology for the 21st century. Curr Opin Chem Biol,2000,4 445～451.
  • 6[6]Gonzalez JE, Oades K, leychkis Y, et al. Cell-based assays and instrumentation for screening ion-channel targets. Drug Discov Today,1999,4 431～439.
  • 7[7]Taylor DL, Woo ES, Giuliano KA. Real-time molecular and cellular analysis: the new frontier of drug discovery. Curr Opin Biotechnol,2001,12 75～81.
  • 8[8]Kain SR. Green fluorescent protein (GFP): applications in cell-based assays for drug discovery. Drug Discov Today,1999,4 304～312.
  • 9[9]Ramm P. Imaging systems in assay screening. Drug Discov Today,1999,4 401～410.
  • 10[10]Drews J. Drug discovery: a historical perspective. Science,2000,287 1960～1964.

共引文献18

同被引文献21

  • 1张琦,魏尔清,朱朝阳,张世红,张纬萍,俞月萍,陈忠.一种测定小鼠长时间自发活动时空特点的新方法[J].浙江大学学报（医学版）,2004,33(6):509-514. 被引量：9
  • 2胡欣,葛求富,张磊,卢韵碧,魏尔清.依达拉奉抗神经元缺血性损伤作用的内环境影响因素[J].浙江大学学报（医学版）,2006,35(2):147-153. 被引量：4
  • 3盛雯雯,张纬萍,王梦令,张世红,胡华,储利胜,周宇,方三华,余国良,钱晓东,陈科达,徐慧敏,刘路英,张磊,魏尔清.米诺环素对大鼠和小鼠脑外伤的部分保护作用[J].浙江大学学报（医学版）,2006,35(4):411-418. 被引量：2
  • 4BEDERSON J B,PITTS L H,TSUJI M,et al.Rat middle cerebral artery occlusion: evaluation of the model and development of a neurologic examination [J]. Stroke, 1986,17(3) :472-476.
  • 5ZHANGQ,WEI E Q, ZHU C Y,et al. Focal cerebral ischemia alters the spatio-temporal properties ,but not the amount of activity in mice[J]. Behav Brain Res,2006,169(1) :66-74.
  • 6LI X, BLIZZARD K K, ZENG Z, et al. Chronic behavioral testing after focal ischemia in the mouse: functional recovery and the effects of gender [J]. Exp Neurol, 2004,187 (1) : 94-104.
  • 7BUITRAGO M M, SCHULZ J B, DICHGANS J,et al. Short and long-term motor skill learning in an aceelerated rotarod training paradigm [J].Neurobiol Learn Mere, 2004,81 (3) : 211-216.
  • 8ZHANG L, SCHALLERT T, ZHANG Z G, et al. A test for detecting long-term sensorimotor dysfunction in the mouse after focal cerebral ischemia [J].J Neurosei Methods, 2002,117 (2) :207-214.
  • 9WHISHAW I Q, O'CONNOR W TDUNNETT S B. The contributions of motor cortex,nigrostriatal dopamine and caudate-putamen to skilled forelimb use in the rat [J]. Brain, 1986,109(Pt 5):805-843.
  • 10GHARBAWIE O A, GONZALEZ C L,WILLIAMS P T, et al. Middle cerebral artery(MCA) stroke produces dysfunction in adjacent motor cortex as detected by intracortical microstimulation in rats [J]. Neuroseienee, 2005,130(3) : 601-610.

引证文献1

二级引证文献2

浙江大学学报（医学版）
2002年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部