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致敏后重复抗原攻击的大鼠肺组织环核苷酸磷酸二酯酶活性的变化 被引量：3

Cyclic nucleotides phosphodiesterase activity in a rat lung model of asthma
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摘要 目的 :探讨致敏后重复抗原攻击大鼠肺组织 c GMP- PDE、c AMP- PDE活性变化。方法 :HPL C法测定致敏后重复抗原攻击的大鼠 (抗原攻击组 )、致敏不攻击的大鼠 (致敏组 )和正常大鼠 (正常组 )肺组织 c GMP- PDE、c AMP- PDE活性。结果 :抗原攻击组肺组织 c GMP- PDE、c AMP- PDE活性分别为 798.76± 5 2 9.2 3、5 97.5 0± 2 2 0 .75nmol·g-1protein·min-1,其中抗原攻击组大鼠肺组织 c GMP- PDE活性较正常组明显升高 (P<0 .0 5 ) ,抗原攻击组大鼠肺组织 c AMP- PDE活性较致敏组和正常组均明显升高 (P<0 .0 5 )。结论 :大鼠哮喘发病过程中肺组织c GMP- PDE、c AMP- Objective: To assess the change of cGMP PDE and cAMP PDE activity in a rat lung model of asthma. Methods: cGMP PDE and cAMP PDE activity were determined by HPLC. Results: Both cAMP PDE activity and cGMP PDE activity in the lung tissue of antigen challenged rats were higher than that from the normal rats( P <0.05). Conclusion: In the rat lung model of asthma, cyclic nucleotides phosphodiesterase activity was elevated. This may be significant in the pathogenesis of asthma.
作者 宋燕华 陈季强 周汉良
机构地区 浙江大学医学院
出处 《浙江大学学报（医学版）》 CAS CSCD 2002年第2期127-130,共4页 Journal of Zhejiang University(Medical Sciences)
基金 国家自然科学基金资助项目 (39970 85 7)
关键词 环核苷酸磷酸二酯酶 哮喘 病因 色谱法 肺组织 动物实验 HPLC法 cyclic nucleotides phosphodiesterase/metab Asthma/etiol Chromatography,high pressure liquid
分类号 R392.3 [医药卫生—免疫学]
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参考文献10

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同被引文献27

  • 1宋燕华,陈季强,陈俊春.卡介苗对致敏后重复抗原攻击的大鼠肺组织环核苷酸磷酸二酯酶活性的影响[J].中国药理学通报,2005,21(8):1020-1021. 被引量：1
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浙江大学学报（医学版）
2002年 第2期

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