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X射线对小鼠脾细胞内P16、cyclinD1和CDK4表达的影响 被引量:2

Effect of ionizing radiation on expression of P16 cyclin D1 and CDK4 in splenocyctes in mice
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摘要 目的 :探讨 X射线对小鼠脾细胞内 P1 6、cyclin D1和 CDK4表达的影响。方法 :采用细胞间接荧光标记法、FACScan流式细胞仪检测不同剂量 X射线全身照射后小鼠脾细胞内 P1 6、cyclinD1和 CDK4三种蛋白表达的时程变化和量效关系。结果 :2 .0 Gy X射线照后 2 4 h小鼠脾细胞 P1 6表达明显高于假照组 ;CDK4表达水平在照后 8h明显降低 ,持续至照后 72 h仍低于正常水平。周期蛋白 cyclin D1表达轻度下调。不同剂量照射后 P1 6蛋白表达呈剂量依赖性增加 ,1 .0~ 4.0 Gy组与假照射组相比显著增多 ( P<0 .0 5 ,P<0 .0 1 ) ;cyclin D1蛋白表达随剂量升高有降低趋势 ;CDK4亦呈剂量依赖性降低 ,0 .5~ 6.0 Gy组与假照组比较差异具有显著性 ( P<0 .0 5 ,P<0 .0 1 )。结论 :P1 6/cyclin D1 /CDK4/p Rb通路在电离辐射诱导脾细胞 Objective: To determine and analyze the changes in expression in different doses and different time intervals of P16? cyclin D1 and CDK4, respectively, in splenocytes in mice after whole body irradiation. Methods: Indirect fluorescence labble technique and flow cytometry (FCM) was used in the examing. Results: The results showed that protein P16 of splenocytes elevated significantly at 24 h after 2.0 Gy X irradiation in compared with that of sham irradiation. CDK4 protein expression was dramatically decreased at 8 h lasted to 72 h, after 2.0 Gy X rays. cyclinD1 protein showed a little reduction after 2.0 Gy X irradiation. Overexpression of P16 depended on the doses of radiation. The expressions of P16 in 1.0~4.0 Gy group were significantly increased in comparison with that in sham irradiated group ( P<0.05, P <0.01 ). cyclin D1 expression showed declining tendency after different doses X irradiation. The reduction of CDK4 expression was significant in 0.5~6.0 Gy group ( P<0.05, P <0.01), and CDK4 was decreasing with X rays doses increasing. Conclusion:These results suggeste that P16/cyclin D1/CDK4/pRb pathway may play an important role in G 1 arrest of splenocytes induced by ionizing radiation.
出处 《吉林大学学报(医学版)》 CAS CSCD 北大核心 2002年第2期118-120,共3页 Journal of Jilin University:Medicine Edition
基金 国家自然科学基金资助课题 (39770 193)
关键词 电离辐射 P16 CYCLIN D1 CDK4 X射线 动物实验 脾细胞 ionizing radiation P16 cyclin D1 CDK4
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  • 1Sigurdson AJ, Bondy ML, Hess KR,et al. Gamma-ray mutagen sensitivity and survival in patients with glioma[J]. Clin Caucer Res, 1998,4: 3031-3035.
  • 2Klassem NV, Walker PR, Ross CK, et al.Two-stage cell shrinkage and the OER for radiation-induced apoptosis of rat thymoytes[J]. Int J Radial Biol, 1993, 64: 571-581.
  • 3Barker F, Chen P, Furman F, et al. p16 deletion and mutation analysis in human brain tumors[J]. J Neurooncol, 1997, 31:17-23.
  • 4Hama S, Heike Y, Takahashi M, et al. Adenovirous-mediated p16 gene transfer drug-induced cell death through G1 arrest in human glioma cell. Int J Cancer, 1998, 77:47-49.
  • 5Bernhard EJ, Maity A, Muschel RJ, et al . Effects of ionizing radiation on cell cycle progression[J]. Radiat Environ Biophys,1995,34(2):79-83.
  • 6Robert AW. The retinoblastoma protein and cell cycle control cell. Am J Respir Crit Care Med,1995,81:323-330.
  • 7Chakrabarty A, Bridges LR, Gray S, et al . Cyclin D1 in astrocytic tumours: an immunohistochemical study[J]. Neuropathol Appl Neurobiol,1996,22(4): 311-316.
  • 8Schmidt EE, Ichimura K, Reifenberger G, et al . CDKN2(P16/MTS1) gene deletion or CDK4 amplification occurs in the majority of glioblastomas[J].Cancer Res,1994,54(24):6 321-6 324.
  • 9Nakamura M, Konishi N, Tsunoda S, et al .Retinoblastoma protein expression and MIB-1 correlate with survival of patients with malignant atrocytoma[J].Cancer,1997,80(2):242-249.
  • 10Chazal M, Marionnet C, Michel L, et al .P16(INK4A) is implicated in both the immediate and adaptive response of human keratinocytes to UVB irration[J].Oncogene, 2002,18,21(17):2 652-2 661 .

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