金龟子绿僵菌室内侵染刚竹毒蛾试验

Studies on Laboratory Infection of Metarhizium anisopliae to Pantana phyllostachysae

3个金龟子绿僵菌菌株 ( M1、M2 、M3 )经室内侵染试验证明 ,皆能侵染 2～ 3龄刚竹毒蛾幼虫 ,其中以 M3 菌株的致病力较强 ,M1和 M2 次之 .M3 的 L C50 =7.5 5× 1 0 6孢子· L-1,L T50 =5 .88～ 1 0 .5 0 d(在 1 .0× 1 0 11～ 1 .0× 1 0 7孢子· L-1浓度下 ) .M1和 M2 的 LC50 分别为 3.1 2× 1 0 7孢子·L-1和 7.0 1× 1 0 7孢子· L-1,LT50 分别为 6.5 0～ 1 1 .65 d和 7.66～ 1 1 .5 3d.不同温度、湿度下的分生孢子萌发力试验表明 ,供试的 3个菌株具有较强的耐高温和耐旱特性 .

strains of Metarhizium anisopliae(Metsch.) Sorokin infected Pantana phyllostachysae Chao in laboratory,and all showed pathogenicity to 2nd^3rd instar larvae of P.phyllostachysae.Of 3 strains,M 3 caused the greatest mortality,with M 1 and M 2 next to it.The LC 50 of M 3 was 7 55×10 6 spores· L -1 ,and the LT 50 was 5 88~10 50 days for the concentration of 1 0×10 11 ~1 0×10 7 spores·L -1 The LC 50 of M 1 and M 2 were 3 12×10 7 spores·L -1 ,7 01×10 7 spores· L -1 ,and the LT 50 were 6 50~11 65 days,7 66~11 53 days respectively The experiment of conidial germination was carried out at different temperature and in different humidity,and showed greater resistance to high temperature and drought The laboratory test indicated that M anisopliae was of greater value in controlling P phyllostachysae