热休克蛋白70大鼠脑弥漫性轴索损伤后神经元保护及修复作用研究

Investigations on neuron protection and repairing effect of heat shock protein 70 after diffuse axis-cylinder injury of rats

目的探讨大鼠脑弥漫性轴索损伤(diffuseaxonalinjuries,DAI)后不同时期脑皮层组织中热休克蛋白(heatshockproteins,HSP)70的表达,为临床治疗脑损伤寻求有效手段。方法制作大鼠DAI模型,分别在伤后6、24、48、72h,7d以免疫组织化学方法检测大鼠脑皮层成纤维细胞生长因子(bFGF)的表达,分析其含量变化特点。结果DAI后24hHSP表达增加,48h达高峰,7d后仍维持较高水平。结论bFGF在各时间点表达有明显差异,提示其参与脑损伤后神经元保护及修复过程。

Objective To study the expression and its roles of HSP in the cerebral contex of rats with diffuse axonal injuries in order to find a new method to treat brain injuries.Methods Diffuse axonal injuries were produced by DAI model. And the changes of bFGF expression in cerebral contex were detected by immunohistochemistry from 6 h to 7 d after injuries.Results The expression of HSP was increased at 24 h after DAI(P< 0.01).And its expression reached the highest level at 48h(P< 0.01),and still in a high level at 7d after injuries(P< 0.01).Conclusion Statistical analysis showed a significant difference in the expression of HSP after DAI in rats,which suggests that HSP plays an important role in the neuronal protection and repair after brain injuries.