摘要
本文首次以广东禽流感无致病力分离株A/goose/China/24/96（H7N3）核蛋白（NP）基因核酸为模板,采用逆转录聚合酶链反应（RT-PCR）技术,扩增出了预计的830 bp左右的片段。将此扩增产物克隆进pGEM-4Z载体,采用限制性内切酶、地高辛标记探针和序列测定对该重组质粒进行了鉴定。测出的NP序列经Blast2.0比较,与GenBank中收录的AIV其它病毒株NP基因相应片段的同源性在91％以上。
Conservative code region (about 830bp) of the nucleoprotein ( NP) sequences from avian influenza H7N3 strain was amplified by reverse transcription polymerase chain reaction (RT-PCR) and ligased with the pGEM-4Z,the recombiant plasmids containing the fragment about 830bp were identified by restriction endonu-clease analysis and PCR DIG probe and analysis of sequence respectively. It is suggested that the homology of the sequence of nucleoprotein gene of strain H7N3 is more than 91 % comparing with other strains of avian influenza virus.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2002年第2期165-168,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
广东省"九五"重点攻关项目(199603704)
国家973项目资助(G1999011900)。
