东北地区部分玉米品种资源抗玉米矮花叶病鉴定研究

Studies on Evaluation of Resistance of Maize Germplasms to MDMV-B in Northeastern China

以田间人工接种玉米矮花叶病毒 (MDMV -B)和自然感染相结合方法 ,鉴定了东北地区部分玉米品种资源 10 0份 ,筛选出 5 4 3、2 12 5、2 0 2 6、CY119等自交系和沈试 2 9、87_1×L10 5、4 4 4× 87_1、丹 4 0 8_2、吉单 3 2 1等杂交种高抗材料 13份。对部分玉米材料以温室苗期接毒鉴定与大田接毒鉴定比较 ,多数品种温室鉴定比田间鉴定发病率高 1倍左右 ,但部分品种仍表现出与田间一致的抗病性。温室鉴定应该与大田鉴定相结合以缩短鉴定年限 ,使鉴定结果更加可靠。大田鉴定播期不同 ,同一品种的抗病性表现有别 ,播期晚病率高、病情重 ;接毒后抗性品种潜育期长。

Resistance of 100 maize germplasms to the strain B of maize dwarf mosaic virus(MDMV_B) was evaluated by artificial inoculated and natural infection in the field.Among them,13 maize lines were resistant,they are:543,2125,2026,CY119,Shenshi29,87_1×L105,444×87_1,Dan408_2 and Jidan321.Comparison of resistance assay for some maize lines was conducted by inoculated at seedling in greenhouse and at adult in field ,the incidence in greenhouse appears to be more about one time than that in field ,however,the resistance for few maize lines in greenhouse was consistent with that in field.The evaluation for resistance inoculated in the field should be combined with the evaluation in greenhouse ,so that the evaluation may be quick and reliable.The resistance appearance of maize germplasms should be different at different sowing time in the field for a same maize line,the later they were sowed,the severer they were diseased.The incubative stage was longer and the symptom emerged slower in resistant maize lines.