青海东部黄牛血液生化遗传多态性

Investigation of Polymorphism of Biochemical Genetic in Blood for Qinghai East Yellow Cattle

采用聚丙烯酰胺凝胶电泳法和火焰光度法 ,对青海东部黄牛的HB ,KE ,TF ,PTF ,PA ,AMY 1,ALPA ,ALPB ,RBC LDH1,S LDH110个血液生化遗传基因座的多态性进行了研究。结果发现 :( 1)在被研究的 10个生化遗传基因座中有 8个存在多态性 ,多态性基因座比例为 80 .0 % ;( 2 )各生化遗传基因座的优势等位基因是 :HBA,KEL,TFD,PTFB,PAB,AMY1B,ALPAA,ALPB0 ,RBC LDH1S,S LDH1S;( 3)平均基因杂合度和基因纯合度指数分别为 0 .30 7和 0 .5 39,实有等位基因数和有效等位基因数分别为 2 .40 0和 1.5 92个。

The polymorphism of ten biochemical genetic loci including HB,KE,TF,PTF,PA,AMY 1,ALP A,ALP B,RBC-LDH 1,S-LDH 1 for Qinghai east yellow cattle were investigated by polyacrylamide gel electrophoresis and flame photometer.The results were as follows:(1)There was a polymorphism in eight of ten biochemical genetic loci investigeted and the proportion of polymorphic loci was 80.0%.(2)The preponderant alleles on the loci of these biochemical genetic markers were HB A,KE L,TF D,PTF B,PA B,AMY1 B,ALP A A,ALP B 0,RBC-LDH1 S,S-LDH1 S.(3)The average gene heteroxygosity and gene homozygosity index were 0.3072 and 0.5389,resp.The average numbers of real and effective allele were 2.400 and 1.5922,resp.