百合多糖的纯化与化学结构鉴定研究

Isolation and Purification of Polysaccharides from Lily

本研究从百合水提液中分离纯化得到两种多糖LP1、LP2,得率分别为鲜百合重0.55%、0.25%,经纸层析和凝胶柱层析鉴定均为单一组分,将这两种多糖进行气相色谱和凝胶柱层析分析,结果如下:LP1由葡萄糖、甘露糖组成,比例为1:2.46,分子量为7.94×104LP2由葡萄糖、半乳糖、甘露糖、阿拉伯糖、半乳糖醛酸组成,比例为1:0.73:2.6:1.8:0.84,分子量1.

Two polysaccharides are isolated from lily.The polysaccharides (LP1、LP2) are separated after water abstract lily-polysaccharide was eluted from DEAE-cellulose column,and make up 0.55 and 0.25 percent of fresh lily weight respectively.LP1 and LP2 are pure polysaccharides by appraised of purity.The chemical components and the molecule weight of the two polysaccharides are appraised by gas chromatography and chromatography on Sephadex G-200 column,respectively;resultes are:LP1's sugar composition comprises:Glu.、Man.With a rate of 1:2.46, and its molecule weight is 79400.LP2's sugar composition comprises: Glu.、Gal.、Man.、Ara.and Gal.Acid with a rate of 1:0.73:2.6:1.8:0.84,and its molecular weight is 18150.