外源水杨酸对辣椒倍半萜环化酶基因表达及抗氧化酶系的作用

Induction of sesquiterpene cyclase gene expression and antioxidant enzyems regulated by exongenous salicylic acid in leaves of Capsicum annuum.

分析了外源水杨酸对辣椒叶片倍半萜环化酶基因表达及抗氧化酶系的作用 .结果表明 ,在 0 .5～4mmol·L-1的浓度范围内 ,SA处理均能不同程度地诱导辣椒叶片中倍半萜环化酶基因转录并表达酶活性 ,但是酶活性较低且在SA处理 36h后才出现 ;SA处理后 ,辣椒叶片SOD和POD酶活性较对照增高 ,CAT酶活性较对照降低 ,相应地 ,H2 O2 浓度升高 .H2 O2

An analysis of the induction of sesquiterpene cyclase gene expression and antioxidant enzymes in Capsicum annuum by exongenous salicylic acid (SA) pointed out that the sesquiterpene cyclase gene expression in detached pepper leaves was induced by 0\^5～4mmol·L -1 of exongenous SA,whereas the enzyme activity and accumulation of cyclase mRNA were much lower,and it took a longer time (36h) for the gene to be induced to express after SA treatment,comparing to other elicitors such as UV and fungi treatment.The activities of SOD and POD were enhanced,while the CAT activity was inhibited to some degree. Consequently,H\-2O\-2 content was increased in SA treated pepper leaves.H\-2O\-2 accumulation was related to the integrated influences from antioxidant enzymes.