六亚甲基二乙酰胺对粘液表皮样癌细胞P^(21)和P^(53)基因的转录激活作用

The effect of hexamethylene bisacetamide(HMBA)on activating transcription P^(21)(cip1/waf1) and P^(53) in mucoepidermoid carcinoma cell in vitro

为了研究分化诱导剂六亚甲基二乙酰胺 (hexamethylene bisacetamide,HMBA)在体外诱导人粘液表皮样癌细胞 (MEC- 1)时 P2 1(cip1/ waf1) ,P53 基因调控的机理 ,从而为临床治疗提供理论依据 .选用 0 .0 0 2 mol/ L HMBA和 0 .0 0 1g/ L 5 - FU对培养的 MEC- 1细胞体外诱导 72 h后 ,分别采用光镜、TUNEL染色、免疫组化、图像分析等方法进行凋亡细胞的检测及 P2 1(cip1/ waf1) ,P53表达的定量分析 .结果表明 HMBA诱导的 MEC- 1细胞 P2 1(cip1/ waf1)的表达强度显著高于对照组 (P <0 .0 1) ,P53 的表达强度显著低于对照组 (P <0 .0 1) .提示 HMBA通过激活转录因子 P2 1(cip1/ waf1) ,P53的表达而发挥对粘液表皮样癌细胞诱导分化。

To investigate the effects of hexamethylene bisacetamide(HMBA) on apoptosis and the expression of P 21(cip1/waf1) and P 53 in human mucoepidermoid carcinoma cell line MEC-1.MEC-1 was induced 72 hours by 0.002 mol/L HMBA and 0.001 g/L 5-Flourouracil(5-FU) in vitro. Apoptosis index,the expression of P 21(cip1/waf1) and P 53 protein were determined by morphologic observation, terminal dUTP nick end labeling(TUNEL) strain, immunohistochemistry, image analysis.The expression of P 21(cip1/waf1) in HMBA-treated MEC-1 was markedly higher than that of the non- HMBA-treated;but the expression of P 53 was decreased by HMBA-treated.There were significant difference between the treated and non-treated groups(P<0.001). HMBA could induce marked apoptosis and potentiated differentiation in human mucoepidermoid carcinoma cell line .The mechanism could be activating transcription regulation of P 21(cip1/waf1) and P 53 genes.